Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Salmonella Enteritidis Exposure to Acid Stress.
Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Salmonella Enteritidis Exposure to Acid Stress.
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酸胁迫下肠炎沙门氏菌成熟生物膜和浮游细胞的初步转录组分析
DOI：
10.3389/fmicb.2017.01861
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发表时间：
2017
影响因子：
5.2
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Jia K;Wang G;Liang L;Wang M;Wang H;Xu X
沙门氏菌已成为一种公认的食源性病原体，许多菌株能够形成生物膜，从而在广泛遇到酸基消毒剂的食品加工环境中造成交叉污染。在本研究中，采用 RNA 测序来建立肠炎沙门氏菌在胰蛋白酶大豆胨肉汤 (TSB) 和酸性 TSB (aTSB) 中培养的浮游细胞和生物膜相关细胞形式的完整转录组谱。在相同条件下培养的生物膜相关细胞和浮游细胞之间，肠炎沙门氏菌的基因表达模式存在显着差异。本研究中肠炎沙门氏菌的组装转录组包含 5,442 个组装转录本，其中包括在生物膜和浮游细胞中鉴定的 3,877 个差异表达基因 (DEG)。这些DEG在生物过程、代谢过程、大分子复合物、结合和转移酶活性的调节等方面得到丰富，可能在aTSB培养的肠炎沙门氏菌生物膜形成中发挥关键作用。观察到三个重要途径在酸性条件下富集：细菌趋化性、卟啉-叶绿素代谢和硫代谢。此外，还鉴定了 15 种差异表达的新型非编码小 RNA (sRNA)，并且仅发现一种在成熟生物膜中上调。这项对肠炎沙门氏菌转录组的初步研究为未来研究调节酸性环境中沙门氏菌生物膜的复杂网络系统奠定了基础，提供了有关生物膜形成和酸应激相互作用的信息，可能有助于食品加工行业新型消毒程序的开发。
Salmonella has emerged as a well-recognized food-borne pathogen, with many strains able to form biofilms and thus cause cross-contamination in food processing environments where acid-based disinfectants are widely encountered. In the present study, RNA sequencing was employed to establish complete transcriptome profiles of Salmonella Enteritidis in the forms of planktonic and biofilm-associated cells cultured in Tryptic Soytone Broth (TSB) and acidic TSB (aTSB). The gene expression patterns of S. Enteritidis significantly differed between biofilm-associated and planktonic cells cultivated under the same conditions. The assembled transcriptome of S. Enteritidis in this study contained 5,442 assembled transcripts, including 3,877 differentially expressed genes (DEGs) identified in biofilm and planktonic cells. These DEGs were enriched in terms such as regulation of biological process, metabolic process, macromolecular complex, binding and transferase activity, which may play crucial roles in the biofilm formation of S. Enteritidis cultivated in aTSB. Three significant pathways were observed to be enriched under acidic conditions: bacterial chemotaxis, porphyrin-chlorophyll metabolism and sulfur metabolism. In addition, 15 differentially expressed novel non-coding small RNAs (sRNAs) were identified, and only one was found to be up-regulated in mature biofilms. This preliminary study of the S. Enteritidis transcriptome serves as a basis for future investigations examining the complex network systems that regulate Salmonella biofilm in acidic environments, which provide information on biofilm formation and acid stress interaction that may facilitate the development of novel disinfection procedures in the food processing industry.