Detection of a Novel Astrovirus in Brain Tissue of Mink Suffering from Shaking Mink Syndrome by Use of Viral Metagenomics
Detection of a Novel Astrovirus in Brain Tissue of Mink Suffering from Shaking Mink Syndrome by Use of Viral Metagenomics
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DOI：
10.1128/jcm.01040-10
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发表时间：
2010-12-01
影响因子：
9.4
通讯作者：
Berg, Mikael
中科院分区：
文献类型：
--
作者：
Blomstrom, Anne-Lie;Widen, Frederik;Berg, Mikael
2000年，丹麦养殖的水貂患上了一种神经系统疾病。特征性临床体征包括颤抖、蹒跚步态和共济失调。这种疾病被命名为抖貂综合征，通过将患病水貂的脑匀浆接种到健康水貂身上来复制。然而，病因仍然不明，尽管密集的努力。本研究采用随机扩增和大规模测序的方法，在3只实验感染水貂的脑组织中检测到一种星状病毒。这种病毒也在三只自然表现出这种疾病的水貂的大脑中发现，但在六只健康的动物中没有发现。对检测到的星状病毒的完整编码区进行测序，并与断奶前腹泻相关的水貂星状病毒和最近发现的与X连锁无丙种球蛋白血症男孩脑炎病例相关的人类星状病毒进行比较。同源性分别为80.4%和52.3%，表明本研究中描述的病毒与断奶前腹泻水貂星状病毒更相似。对于非结构编码区的序列同一性是约90%相比，星状病毒，这是与断奶前腹泻水貂。编码结构蛋白的区域更加多样化，仅显示出67%的序列同一性。这一发现之所以令人感兴趣，不仅是因为检测到的病毒可能是水貂颤抖综合征的病原体，还因为这是首次描述在动物中枢神经系统中发现的星状病毒之一。
In 2000, farmed mink kits in Denmark were affected by a neurological disorder. The characteristic clinical signs included shaking, staggering gait, and ataxia. The disease, given the name shaking mink syndrome, was reproduced by the inoculation of brain homogenate from affected mink kits into healthy ones. However, the etiology remained unknown despite intensive efforts. In this study, random amplification and large-scale sequencing were used, and an astrovirus was detected in the brain tissue of three experimentally infected mink kits. This virus also was found in the brain of three mink kits naturally displaying the disease but not in the six healthy animals investigated. The complete coding region of the detected astrovirus was sequenced and compared to those of both a mink astrovirus associated with preweaning diarrhea and to a recently discovered human astrovirus associated with a case of encephalitis in a boy with x-linked agammaglobulinemia. The identities were 80.4 and 52.3%, respectively, showing that the virus described in this study was more similar to the preweaning diarrhea mink astrovirus. For the nonstructural coding regions the sequence identity was around 90% compared to that of the astrovirus, which is associated with preweaning diarrhea in mink. The region coding for the structural protein was more diverse, showing only 67% sequence identity. This finding is of interest not only because the detected virus may be the etiological agent of the shaking mink syndrome but also because this is one of the first descriptions of an astrovirus found in the central nervous system of animals.