Insertion of lipid domains into plasma membranes by fusion with erythrocytes.
Insertion of lipid domains into plasma membranes by fusion with erythrocytes.
复制标题
通过与红细胞融合将脂质结构域插入质膜。
DOI：
10.1016/0167-4889(85)90070-9
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Williamson,P
中科院分区：
文献类型：
--
作者：
Schlegel,RA;Lumley-Sapanski,K;Williamson,P
用几种脂质特异性荧光探针预标记质膜后，使用聚乙二醇或仙台病毒作为融合剂将具有对称脂质双层的红细胞与培养细胞融合。一些非特异性探针在融合后被转移到培养细胞膜上并均匀分布在培养细胞膜内。相比之下，当使用部花青 540（其显示优先结合脂质松散堆积的双层）来预标记红细胞时，即使在融合后 24 小时，荧光仍然局限于膜的一个小限制区域内。这些结果表明，将红细胞膜的脂质插入培养细胞的质膜中可以产生离散的结构域，这些结构域在融合后长时间持续存在。因为红细胞膜的插入蛋白类似地不会自由地扩散到整个培养细胞膜，所以膜蛋白和脂质之间的相互作用可能涉及这种单一的区室化。
After prelabeling the plasma membrane with several lipid-specific fluorescent probes, erythrocytes with symmetric lipid bilayers were fused with culture cells using either poly(ethylene glycol) or Sendai virus as fusogen. Several nonspecific probes were transferred to, and became uniformly distributed within, the culture cell membrane upon fusion. In contrast, when merocyanine 540, which displays preferential binding to bilayers in which the lipids are loosely packed, was used to prelabel erythrocytes, fluorescence remained localized within a small confined area of the membrane, even 24 h after fusion. These results suggest that insertion of the lipids of the erythrocyte membrane into the plasma membrane of the culture cell can produce discrete domains which persist as such for long periods following fusion. Because the inserted proteins of the erythrocyte membrane similarly do not freely diffuse throughout the culture cell membrane, interactions between membrane proteins and lipids may be involved in this singular compartmentalization.