Distinct Populations of presympathetic premotor neurons express orexin or melanin-concentrating hormone in the rat lateral hypothalamus
Distinct Populations of presympathetic premotor neurons express orexin or melanin-concentrating hormone in the rat lateral hypothalamus
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DOI：
10.1002/cne.21511
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发表时间：
2007-12-10
影响因子：
2.5
通讯作者：
Watson, Stanley J.
中科院分区：
文献类型：
--
作者：
Kerman, Ilan A.;Bernard, Rene;Watson, Stanley J.
食欲素和黑色素浓集激素（MCH）已被牵连在介导各种不同的行为。这些包括睡眠和觉醒，运动，摄食行为和战斗或逃跑反应，以及啮齿动物的焦虑和恐慌样行为。尽管有这样的多样性，所有这些过程需要协调招聘的自主和somatomotor传出。我们以前绘制的位置，前交感神经运动前神经元（PSPMNs）在大鼠大脑。这些假定的双功能神经元发送跨突触投射到躯体运动和交感神经靶点，并可能参与躯体运动-交感神经整合。这些神经元的显著部分存在于背内侧（DMH）和外侧下丘脑（LH）内，这是在中枢神经系统中含有MCH和食欲素合成神经元的脑区域。因此，我们假设下丘脑PSPMNs利用MCH或食欲素作为其神经递质。为了验证这一假设，我们通过使用伪狂犬病病毒（PRV）重组株进行跨突触束追踪来鉴定PSPMNs。将PRV-152（一种表达增强的绿色荧光蛋白的菌株）注射到交感神经切除的腓肠肌中，而将表达β-半乳糖苷酶的PRV-BaBlu注射到相同动物的肾上腺中。通过使用免疫荧光方法，我们确定是否共同感染的神经元表达MCH或食欲素。我们的研究结果表明，PSPMNs合成MCH或食欲素存在于LH中，它们形成两个独立的群体。位于穹窿周围的PSPMN表达食欲素，而位于大脑脚周围的PSPMN更可能表达MCH。LH内的这两组PSPMNs可能在自主稳态和压力应对机制中发挥不同的功能作用。J. Comp.神经元505：586-601，2007. (c)2007 Wiley-Liss，Inc.
Orexin and melanin-concentrating hormone (MCH) have been implicated in mediating a variety of different behaviors. These include sleep and wakefulness, locomotion, ingestive behaviors, and fight-or-flight response, as well as anxiety- and panic-like behaviors in rodents. Despite such diversity, all these processes require coordinated recruitment of the autonomic and somatomotor efferents. We have previously mapped the locations of presympathetic-premotor neurons (PSPMNs) in the rat brain. These putative dual-function neurons send trans-synaptic projections to somatomotor and sympathetic targets and likely participate in somatomotor-sympathetic integration. A significant portion of these neurons is found within the dorsomedial (DMH) and lateral hypothalamus (LH), areas of the brain that contain MCH- and orexin- synthesizing neurons in the central nervous system. Thus, we hypothesized that hypothalamic PSPMNs utilize MCH or orexin as their neurotransmitter. To test this hypothesis, we identified PSPMNs by using recombinant strains of the pseudorabies virus (PRV) for trans-synaptic tract tracing. PRV-152, a strain that expresses enhanced green fluorescent protein, was injected into sympathectomized gastrocnemius muscle, whereas PRV-BaBlu, which expresses beta-galactosidase, was injected into the adrenal gland in the same animals. By using immunofluorescent methods, we determined whether co-infected neurons express MCH or orexin. Our findings demonstrate that PSPMNs synthesizing either MCH or orexin are present within LH, where they form two separate populations. PSPMNs located around the fornix express orexin, whereas those located around the cerebral peduncle are more likely to express MCH. These two clusters of PSPMNs within LH likely play distinct functional roles in autonomic homeostasis and stress coping mechanisms. J. Comp. Neurol. 505:586-601, 2007. (c) 2007 Wiley-Liss, Inc.