Cross-Linked Micellar Spherical Nucleic Acids from Thermoresponsive Templates.
Cross-Linked Micellar Spherical Nucleic Acids from Thermoresponsive Templates.
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DOI：
10.1021/jacs.6b13359
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发表时间：
2017-03-29
影响因子：
15
通讯作者：
Mirkin CA
中科院分区：
文献类型：
--
作者：
Banga RJ;Meckes B;Narayan SP;Sprangers AJ;Nguyen ST;Mirkin CA
报道了以Pluronic F127为热响应模板，一锅法合成了胶束球形核酸（SNA）纳米结构。这些新型结构是通过化学上简单的过程合成的，包括将DNA两亲体的脂质尾部（用于TLR-9刺激的CpG基序）插入Pluronic F127胶束的疏水区域，然后进行化学交联，随后去除非交联结构。交联胶束SNA致密的核酸壳增强了其在生理介质中的稳定性，促进了其快速的细胞内化，使其成为有效的TLR-9免疫调节剂。这些结构强调了sna在调节免疫反应中的潜力，并解决了非共价结构相对缺乏稳定性的问题。
A one-pot synthesis of micellar spherical nucleic acid (SNA) nanostructures using Pluronic F127 as a thermoresponsive template is reported. These novel constructs are synthesized in a chemically straightforward process that involves intercalation of the lipid tails of DNA amphiphiles (CpG motifs for TLR-9 stimulation) into the hydrophobic regions of Pluronic F127 micelles, followed by chemical cross-linking and subsequent removal of non-cross-linked structures. The dense nucleic acid shell of the resulting cross-linked micellar SNA enhances their stability in physiological media and facilitates their rapid cellular internalization, making them effective TLR-9 immunomodulatory agents. These constructs underscore the potential of SNAs in regulating immune response and address the relative lack of stability of noncovalent constructs.