Use of rabbit serum in the cultivation of Plasmodium falciparum.
Use of rabbit serum in the cultivation of Plasmodium falciparum.
复制标题
兔血清在恶性疟原虫培养中的应用。
DOI：
10.2307/3280518
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
K. Rieckmann
中科院分区：
文献类型：
--
作者：
L. Sax;K. Rieckmann
在早先在含人血清的培养基中培养4株恶性疟原虫后，在含兔血清的培养基中进行了长期培养。寄生虫在适应新环境2- 3周后，在新鲜冷冻兔血清中的生长与在人血清中观察到的生长没有区别。此外，在添加5%冻干血清的培养基中，寄生虫的生长情况与添加5%或10%冷冻血清的培养基相似。用不同家兔的血清观察到的寄生虫生长的变异性可以通过汇集血清样本来克服。结果表明，在许多不同的情况下，用兔血清代替人血清培养恶性疟原虫是有帮助的。
Long-term cultivation of four strains of Plasmodium falciparum was achieved in medium with rabbit serum following earlier cultivation of these parasites in medium containing human serum. After a 2- to 3-wk period of adaptation by parasites to their new environment, growth in fresh-frozen rabbit serum was indistinguishable from that observed in human serum. Furthermore, the growth of parasites in medium supplemented with 5% lyophilized serum was similar to that observed when 5 or 10% frozen serum was used. Variability in parasite growth observed with sera obtained from different rabbits could be overcome by the pooling of serum samples. The results suggest that the replacement of human serum by rabbit serum in the cultivation of P. falciparum would be helpful under a number of different circumstances.