Two-year prospective study of single infections and co-infections by respiratory syncytial virus and viruses identified recently in infants with acute respiratory disease
Two-year prospective study of single infections and co-infections by respiratory syncytial virus and viruses identified recently in infants with acute respiratory disease
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DOI：
10.1002/jmv.21108
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发表时间：
2008-04-01
影响因子：
12.7
通讯作者：
Clementi, Massimo
中科院分区：
文献类型：
--
作者：
Canducci, Filippo;Debiaggi, Maurizia;Clementi, Massimo
对意大利北部儿科住院的 322 名急性呼吸道疾病 (ARD) 婴儿患者进行了一项为期 2 年的前瞻性分析，以评估最近发现的病毒作为呼吸道病原体或共病原体的作用。通过分子检测和临床症状评估来检测呼吸道合胞病毒（RSV）、人间质肺病毒（hMPV）、人博卡病毒（hBoV）和人冠状病毒（hCoV）的存在。 322 名婴儿中有 150 名 (46.6%) 的鼻咽抽吸物检测出至少一种病原体呈阳性。 90 个样本 (28.0%) RSV RNA 检测呈阳性（61.5% 基因型 A 和 38.5% 基因型 B），46 个样本（14.3%）hMPV RNA 检测呈阳性（71.7% A 亚型和 28.3% B 亚型），28 个样本（8.7%）hCoV RNA（39.3% hCoV-OC43、35.7% hCoV-NL63、 21.4% hCoV-HKU1 和 3.6% hCoV-229E），7 个（2.2%）hBoV DNA（6 种类型中，50% 亚型 1 和 50% 亚型 2）； 21/150 个样本显示存在 2 种或更多病毒。 hMPV、hCoV 和 hBoV 的共同感染率较高（38.3%、46.4% 和 57.1%），而 RSV 的共同感染率较低（23.3%）。 RSV 与并发症的存在相关（P
A prospective 2-year analysis including 322 infant patients with acute respiratory disease (ARD) hospitalized in a pediatric department in northern Italy was carried out to evaluate the role as respiratory pathogens or co-pathogens of recently identified viruses. The presence of respiratory syncitial virus (RSV), human Metapneumoviruses (hMPVs), human Bocaviruses (hBoVs), and human Coronaviruses (hCoVs) was assayed by molecular detection and clinical symptoms evaluated. Nasopharyngeal aspirates from 150 of the 322 infants (46.6%) tested positive for at least one pathogen. Ninety samples (28.0%) tested positive for RSV RNA (61.5% genotype A and 38.5% genotype B), 46 (14.3%) for hMPV RNA (71.7% subtype A and 28.3% subtype B), 28 (8.7%) for hCoV RNA (39.3% hCoV-OC43, 35.7% hCoV-NL63, 21.4% hCoV-HKU1, and 3.6% hCoV-229E), and 7 (2.2%) for hBoV DNA (of the 6 typed, 50% subtype 1 and 50% subtype 2); 21/150 samples revealed the presence of 2 or more viruses. Co-infection rates were higher for hMPVs, hCoVs, and hBoV (38.3%, 46.4%, and 57.1%,) and lower for RSV (23.3%). RSV was associated with the presence of complications (P