Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae
Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae
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DOI：
10.1111/j.1469-8137.2010.03217.x
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发表时间：
2010-01-01
期刊：
影响因子：
9.4
通讯作者：
Lahaye, Thomas
中科院分区：
文献类型：
--
作者：
Roemer, Patrick;Recht, Sabine;Lahaye, Thomas
P>黄单胞菌属植物病原菌注入类转录激活因子效应蛋白 (TALe)，这些蛋白结合并激活宿主启动子，从而促进疾病或诱导植物防御。 TALes 通过串联排列的 34/35 氨基酸重复序列与相应的 UPT（由 TALe 上调）启动子盒结合。最近的研究揭示了 TALe 代码，其中每个重复的两个氨基酸残基定义了与 UPT 框的特定配对。在这里，我们使用 TALe 代码来预测 TALe 诱导的宿主启动子中潜在的 UPT 框，并通过 β-葡萄糖醛酸酶 (GUS) 报告基因和电泳迁移率变动分析 (EMSA) 进行分析。我们证明来自水稻的 Xa13、OsTFX1 和 Os11N3 启动子是由黄单胞菌直接诱导的米粒细胞株米曲霉 TAL 分别为 PthXo1、PthXo6 和 AvrXa7。我们在每个 TALe 的相应水稻靶启动子中鉴定并功能验证了 UPT 盒，并表明盒突变抑制 TALe 介导的启动子激活。最后，EMSA 证明代码预测的 UPT 盒与相应的 TALes 特异性相互作用。我们的研究结果表明，不同水稻品种的 UPT 盒的变化与这些品种对白叶枯病病原体的易感性或抗性相关。
P>Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes.Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via beta-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA).We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes.Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.