MicroRNA-595 sensitizes ovarian cancer cells to cisplatin by targeting ABCB1.
MicroRNA-595 sensitizes ovarian cancer cells to cisplatin by targeting ABCB1.
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MicroRNA-595 通过靶向 ABCB1 使卵巢癌细胞对顺铂敏感
DOI：
10.18632/oncotarget.13526
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发表时间：
2016-12-27
期刊：
影响因子：
--
通讯作者：
Lou G
中科院分区：
文献类型：
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作者：
Tian S;Zhang M;Chen X;Liu Y;Lou G
卵巢癌是女性与癌症相关死亡的主要原因之一。在这项研究中，我们证明了miR-595表达在卵巢癌组织和细胞系中被下调。淋巴结转移组织中miR-595的表达低于原发性卵巢癌组织和正常组织。此外，miR-595过表达抑制了卵巢癌细胞的增殖，菌落形成和侵袭，并促进了卵巢癌细胞对顺铂的敏感性。我们将ABCB1确定为卵巢癌细胞中miR-595的直接靶基因。在卵巢癌组织和细胞系中，ABCB1表达上调。莫voer，ABCB1的表达水平与卵巢癌组织中的miR-595成反比。此外，ABCB1的过表达降低了miR-595过表达的HO8910PM和SKOV-3细胞对顺铂的敏感性。 ABCB1的异位表达促进了miR-595过表达的HO8910PM和SKOV-3细胞增殖，菌落形成和侵袭。这些数据表明，miR-595在卵巢癌发育中起着抑制肿瘤的作用，并提高了卵巢癌对顺铂的敏感性。
Ovarian cancer is among the leading cause of cancer-related deaths in females. In this study, we demonstrated that miR-595 expression was downregulated in the ovarian cancer tissues and cell lines. miR-595 expression was lower in the lymph node metastases tissues than in the primary ovarian cancer tissues and normal tissues. Furthermore, miR-595 overexpression suppressed the ovarian cancer cell proliferation, colony formation and invasion and promoted the sensitivity of ovarian cancer cell to cisplatin. We identified ABCB1 as a direct target gene of miR-595 in the ovarian cancer cell. ABCB1 expression was upregulated in the ovarian cancer tissues and cell lines. Morevoer, the expression level of ABCB1 was inversely correlated with miR-595 in the ovarian cancer tissues. In addition, overexpression of ABCB1 decreased the miR-595-overexpressing HO8910PM and SKOV-3 cell sensitivity to cisplatin. Ectopic expression of ABCB1 promoted the miR-595-overexpressing HO8910PM and SKOV-3 cell proliferation, colony formation and invasion. These data suggested that miR-595 acted a tumor suppressor role in ovarian cancer development and increased the sensitivity of ovarian cancer to cisplatin.