Oakleaf: an S locus-linked mutation of Primula vulgaris that affects leaf and flower development.
Oakleaf: an S locus-linked mutation of Primula vulgaris that affects leaf and flower development.
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DOI：
10.1111/nph.13370
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Gilmartin PM
中科院分区：
文献类型：
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作者：
Cocker JM;Webster MA;Li J;Wright J;Kaithakottil G;Swarbreck D;Gilmartin PM
在小报春中，异交是通过相互herkogamy与昆虫介导的针形花和线形花之间的异花授粉来促进的。异形花的发育是由S基因座上的基因协调的。为了支持遗传图谱的构建，以促进这些S位点基因的分离，我们已经表征了Oakleaf，一种新的S位点连锁突变表型。我们结合联合收割机的花和叶发育的表型观察，与经典的遗传分析和下一代测序，以解决橡树叶的分子基础。 橡树叶是一种显性突变，影响叶和花的发育;植物产生独特的裂叶，偶尔在静脉异位分生组织。这种表型让人联想到I类KNOX-同源结构域转录因子的过表达。我们描述了野生型和橡树叶植物中所有8个PvKNOX基因的结构和表达，并对橡树叶和野生型植物的叶和花进行了比较转录组分析。 Oakleaf为报春花S基因座的遗传分析提供了一个新的表型标记。我们发现，没有一个I类PvKNOX基因强烈上调橡树叶和花，并确定队列的507上调和314下调基因的橡树叶突变体。
In Primula vulgaris outcrossing is promoted through reciprocal herkogamy with insect‐mediated cross‐pollination between pin and thrum form flowers. Development of heteromorphic flowers is coordinated by genes at the S locus. To underpin construction of a genetic map facilitating isolation of these S locus genes, we have characterised Oakleaf, a novel S locus‐linked mutant phenotype. We combine phenotypic observation of flower and leaf development, with classical genetic analysis and next‐generation sequencing to address the molecular basis of Oakleaf. Oakleaf is a dominant mutation that affects both leaf and flower development; plants produce distinctive lobed leaves, with occasional ectopic meristems on the veins. This phenotype is reminiscent of overexpression of Class I KNOX‐homeodomain transcription factors. We describe the structure and expression of all eight P. vulgaris PvKNOX genes in both wild‐type and Oakleaf plants, and present comparative transcriptome analysis of leaves and flowers from Oakleaf and wild‐type plants. Oakleaf provides a new phenotypic marker for genetic analysis of the Primula S locus. We show that none of the Class I PvKNOX genes are strongly upregulated in Oakleaf leaves and flowers, and identify cohorts of 507 upregulated and 314 downregulated genes in the Oakleaf mutant.