Biogenic phosphorus compounds in sediment and suspended particles in a shallow eutrophic lake: a ³¹P-nuclear magnetic resonance (³¹P NMR) study.
Biogenic phosphorus compounds in sediment and suspended particles in a shallow eutrophic lake: a ³¹P-nuclear magnetic resonance (³¹P NMR) study.
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DOI：
10.1021/es301887z
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发表时间：
2012-09
影响因子：
11.4
通讯作者：
R. Shinohara;A. Imai;N. Kawasaki;Kazuhiro Komatsu;A. Kohzu;S. Miura;T. Sano;Takayuki Satou;N. Tomi
中科院分区：
文献类型：
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作者：
R. Shinohara;A. Imai;N. Kawasaki;Kazuhiro Komatsu;A. Kohzu;S. Miura;T. Sano;Takayuki Satou;N. Tomi
底泥和悬浮物中生物磷化合物的差异对于理解磷的内部负荷机制是重要的，但这些差异仍然是未知的。采用溶态~(31)P-核磁共振(~(31)P)-核磁共振(~(31)P)-NaOH-乙二胺四乙酸乙酯萃取法，检测了葛花源富营养化湖泊悬浮物和沉积物中的多种磷化合物，包括正磷酸盐单酯、正磷双酯、焦磷酸盐和聚磷酸盐。我们验证了这一假设，即在悬浮颗粒和沉积物中，这些组之间存在显著差异。悬浮颗粒物中除正磷酸盐外的其他生物来源磷的比例(占总磷的74.3%)显著高于沉积物(25.6%)。正磷酸盐单酯在悬浮颗粒中的含量相对较高，从正磷酸二酯与单酯的比率(悬浮物平均为0.31；沉积物为1.05)可以看出。经~(31)P核磁共振鉴定为正磷酸单酯的化合物主要来源于磷脂(α-甘油磷酸酯和β-甘油磷酸酯)和核糖核酸(RNAP)，而正磷酸二酯主要是DNA(DNAP)。这些结果表明，正磷双酯的动态、DNA-P的产生或磷脂的降解在鹿儿湖的磷循环中起着重要的作用。
Differences in biogenic phosphorus (P) compounds between sediment and suspended particles in aquatic environments are important for understanding the mechanisms of internal P loading, but these differences are still unknown. We used solution-state (31)P-nuclear magnetic resonance spectroscopy ((31)P NMR) with NaOH-ethylenediaminetetraacetic extraction to detect the multiple P compounds in suspended particles and sediment in the eutrophic Lake Kasumigaura, including orthophosphate monoesters, orthophosphate diesters, pyrophosphate, and polyphosphate. We tested the hypothesis that there is a significant difference between these groups in suspended particles and sediment. Biogenic P other than orthophosphate was found in significantly higher proportions in suspended particles (74.3% of total P) than in sediment (25.6%). Orthophosphate monoesters were comparatively more abundant in suspended particles, as indicated by the ratio of orthophosphate diesters to monoesters (average, 0.31 for suspended particles; 1.05 for sediment). The compounds identified as orthophosphate monoesters by (31)P NMR spectroscopy originated mainly from phospholipids (α-glycerophosphate and β-glycerophosphate) and ribonucleic acid (RNA-P), whereas the orthophosphate diesters included mostly DNA (DNA-P). These results suggest that the dynamics of orthophosphate diesters, the production of DNA-P, or the degradation of phospholipids, play an important role in P cycling in Lake Kasumigaura.