1H NMR spectroscopic imaging of the mouse brain at 9.4 T
1H NMR spectroscopic imaging of the mouse brain at 9.4 T
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DOI：
10.1002/jmri.20709
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发表时间：
2006-10-01
影响因子：
4.4
通讯作者：
Hetherington, Hoby P.
中科院分区：
文献类型：
--
作者：
Miyasaka, Naoyuki;Takahashi, Kan;Hetherington, Hoby P.
目的：材料与方法：对CD-1小鼠进行了21次9.4T H-1光谱成像（SI）研究，包括基底节（N = 5）、海马和丘脑（N = 11）以及小脑（N = 5）。我们使用基于B-0图的全自动匀场计算方法调整了每个切片的B-0均匀性，我们使用具有多个相位演变延迟的多层梯度回波序列测量了该匀场。SI采用了一个修改后的本地化与TE/TR为50/2000毫秒，24 × 24编码的绝热选择性重聚焦（LASER）序列的视场（FOV）为24 mm × 24 mm，1 μ L体素分辨率，和两个平均值，为38 minutes.Results的总采集时间：足够的匀场实现和高质量的光谱一致地获得在每个切片。基底节和丘脑中的N乙酰天冬氨酸（NAA）/肌酸（Cr）比值（分别为0.86 ± 0.07和0.87 ± 0.07）显著高于海马和小脑（0.76 +/- 0.03和0.67 +/- 0.07），它们也彼此显著不同。小鼠脑的H-1 SI具有高度可重复性，并且可以很容易地观察到区域代谢物比率的差异。
Purpose: To assess the feasibility of H-1 spectroscopic imaging (SI) in the mouse brain at 9.4 T, and investigate regional variations in brain metabolites.Materials and Methods: A total of 21 SI studies were performed in CD-1 mice to evaluate the basal ganglia (N = 5), hippocampus and thalamus (N = 11), and cerebellum (N 5). We adjusted the B-0 homogeneity for each slice using a fully automated shim calculation method based on the B-0 map, which we measured using a multislice gradient-echo sequence with multiple phase evolution delays. The SI employed a modified localization by adiabatic selective refocusing (LASER) sequence with TE/TR of 50/2000 msec, 24 X 24 encodes over a field of view (FOV) of 24 mm X 24 mm, 1 mu L voxel resolution, and two averages, for a total acquisition time of 38 minutes.Results: Sufficient shimming was achieved and high-quality spectra were consistently obtained in each slice. Nacetyl aspartate (NAA)/creatine (Cr) ratios in the basal ganglia and thalamus (0.86 +/- 0.07, and 0.87 +/- 0.07, respectively) were significantly higher than those in the hippocampus and cerebellum (0.76 +/- 0.03 and 0.67 +/- 0.07), which were also significantly different from each other.Conclusion: H-1 SI of the mouse brain is highly reproducible and allows differences in regional metabolite ratios to be easily visualized.