Development and variability analysis of microsatellite markers in peach
Development and variability analysis of microsatellite markers in peach
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DOI：
10.1046/j.1439-0523.2002.00656.x
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发表时间：
2002-02-01
期刊：
影响因子：
2
通讯作者：
Arús, P
中科院分区：
文献类型：
--
作者：
Aranzana, MJ;Garcia-Mas, J;Arús, P
以桃品种“梅里尔欧亨利”为材料，构建了富含AG/CT重复序列的基因组DNA文库。富集方法是有效的，61%的克隆携带微卫星序列，每2,17个测序克隆产生一个多态性微卫星。在检测到的35个微卫星中，有24个在25个品种（14个桃和11个油桃）中表现出多态性。共发现82个等位基因的多态性微卫星，平均为37%的杂合性。重复次数多的微卫星通常具有最多的等位基因。除春夫人和皇后外，其它品种都能用所用的标记区分开来，仅用3个微卫星标记就能从25个可能的基因型中区分出24个。聚类分析将所有油桃归为一个簇。桃子的82个等位基因中有75个，比油桃的64个更易变。微卫星标记是桃遗传育种的有效标记。
A genomic DNA library enriched with AG/CT repeats has been developed from the peach cultivar 'Merrill O' Henry'. The enrichment method was efficient, with 61% of the clones obtained carrying a microsatellite sequence and a yield of one polymorphic microsatellite every 2,17 sequenced clones. From 35 microsatellites detected, 24 were polymorphic in a set of 25 cultivars including 14 peaches and 11 nectarines. A total of 82 alleles were found with the polymorphic microsatellites, with an average of a 37% of observed heterozygosity. Microsatellites with a high number of repeats were generally those having the largest number of aleles. All cultivars except two ('Spring Lady' and 'Queencrest') could be individually distinguished with the markers used, Just three selected microsatellites were enough for the discrimination of 24 out of the 25 possible genotypes. Cluster analysis grouped all nectarines in a single cluster. Peaches, with 75 of the 82 alleles found, were more variable than nectarines, with only 64. Microsatellites appear to be powerful and suitable markers for application in peach genetics and breeding.