Effect of N-acetylcysteine on Rat Dental Pulp Cells Cultured on Mineral Trioxide Aggregate
Effect of N-acetylcysteine on Rat Dental Pulp Cells Cultured on Mineral Trioxide Aggregate
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DOI：
10.1016/j.joen.2011.02.012
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发表时间：
2011-05-01
影响因子：
4.2
通讯作者：
Ogawa, Takahiro
中科院分区：
文献类型：
--
作者：
Minamikawa, Hajime;Yamada, Masahiro;Ogawa, Takahiro
简介：本研究的目的是评估矿物三氧化物聚集体（MTA）的细胞毒性和其潜在的解毒抗氧化剂氨基酸，N-乙酰半胱氨酸（NAC）。方法：从大鼠上颌切牙中提取大鼠牙髓细胞，将其直接培养在MTA上，并在培养基中加入NAC。在接种后24小时评估细胞数量和它们的扩散行为。在培养24小时后还评估了活性氧（ROS）和谷胱甘肽（GSH）的细胞内水平。结果：在培养液中加入NAC后，贴附于MTA上的细胞数增加了60%。此外，发现细胞的面积和周长在含有NAC的培养物中大2倍。在单独的MTA上培养的细胞显示出大的ROS浓度，当培养基补充有NAC时其消失。然而，细胞内GSH水平随着NAC的加入而增加3.5倍。结论：本研究表明，NAC在环境中的存在，可以显着改善牙髓细胞在MTA上的附着和扩展行为。这种生物学效应与NAC改善细胞氧化还原系统有关，需要进一步探索NAC以确定其在改善MTA生物相容性方面的治疗价值。（J Endod 2011;37：637-641）
Introduction: The purpose of this study was to evaluate the cytotoxicity of mineral trioxide aggregate (MTA) and its potential detoxification by an antioxidant amino acid, N-acetylcysteine (NAC). Methods: Rat dental pulp cells extracted from rat maxillary incisors were directly cultured on MTA with or without NAC in culture medium. The number of cells and their spreading behavior were both assessed 24 hours after seeding. The intracellular levels of reactive oxygen species (ROS) and glutathione (GSH) were also assessed after 24 hours of culture. Results: The number of cells attached to MTA was 60% greater when NAC was added to the culture medium. In addition, the area and perimeter of the cells were found to be 2-fold greater in the culture containing NAC. Cells cultured on MTA alone showed large ROS concentrations, which disappeared when the medium was supplemented with NAC. The intracellular GSH level, however, increased 3.5-fold with NAC addition. Conclusion's: This study demonstrated that the presence of NAC in environments can substantially improve attachment and spreading behaviors of dental pulp cells on MTA. This biological effect was associated with an im-provement in the cellular redox system by NAC and warrants further exploration of NAC for determining its therapeutic value in improving the biocompatibility of MTA. (J Endod 2011;37:637-641)