Polyaniline-polycaprolactone blended nanofibers for neural cell culture
Polyaniline-polycaprolactone blended nanofibers for neural cell culture
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DOI：
10.1016/j.eurpolymj.2019.04.048
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发表时间：
2019-08-01
影响因子：
6
通讯作者：
Linhardt, Robert J.
中科院分区：
文献类型：
--
作者：
Garrudo, Fabio F. F.;Chapman, Caitlyn A.;Linhardt, Robert J.
神经退行性疾病损害了世界上越来越多的老龄化人口的生活质量。虽然可以诊断，但没有有效的治疗方法。使用组织工程和纳米医学方法，可以开发适合细胞移植的系统。在导电聚合物上培养神经干细胞（NSC）可提高其分化率。本文的研究旨在优化和表征用于神经组织工程应用的 NSC 相容性导电聚己内酯 (PCL)-聚苯胺 (PANI) 电纺支架。此外，理想导电性能所需的最佳 PAM 与 PCL 比例仍不清楚。采用FTIR、TGA和DSC对所得纤维进行了表征，并研究了其材料的力学性能和导电性能。首次在 NSC 中评估 PCL-PANI 纤维的生物相容性；评估细胞粘附、生长速率和形态，并将其与材料的物理化学性质相关联。所有测试的样品都能够支持神经干细胞的生长，而细胞的典型形态不会发生任何重大变化。我们还成功地生产出了电导率高于生物体液的导电纳米纤维（7.7 x 10(-2) S/cm vs 1.0 x 10(-2) S/cm），使其成为电刺激下体外神经分化研究的理想候选者。总体而言，这项研究为改进未来药物测试和组织工程应用的体外模型提供了宝贵的知识。
Neurodegenerative diseases compromise the quality of life of increasing numbers of the world's aging population. While diagnosis is possible, no effective treatments are available. Using both tissue engineering and nanomedicine approaches, it is possible to develop systems appropriated for cell transplantation. Culturing neural stem cells (NSCs) on conductive polymers promotes their differentiation yield. The study herein aims at optimizing and characterizing NSC-compatible, electrically conductive poly(capro-epsilon-lactone) (PCL)-polyaniline (PANI) electrospun scaffolds for neural tissue engineering applications. Furthermore, the optimal PAM to PCL ratio required for ideal electroconductivity properties is still not well understood. The obtained fibers were characterized by FTIR, TGA and DSC, and their material's mechanical properties and electroconductivity, were investigated. For the first time, PCL-PANI fiber's biocompatibility was assessed in NSCs; cell adhesion, growth rate and morphology were evaluated and correlated with the material's physico-chemical properties. All the samples tested were able to support neural stem cell growth without any major changes on the cell's typical morphology. We were also successfully able to produce electrically conductive nanofibers with conductivities above of biological fluids (7.7 x 10(-2) S/cm vs 1.0 x 10(-2) S/cm), making these ideal candidates for in vitro neural differentiation studies under electrical stimulation. Overall, this study provides valuable knowledge to improve future, in vitro models for drug testing and tissue engineering applications.