Notoginsenoside ST-4 inhibits virus penetration of herpes simplex virus in vitro
Notoginsenoside ST-4 inhibits virus penetration of herpes simplex virus in vitro
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DOI：
10.1080/10286020.2011.571645
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发表时间：
2011-01-01
影响因子：
1.7
通讯作者：
Zhang, Ying-Jun
中科院分区：
文献类型：
--
作者：
Pei, Ying;Du, Qian;Zhang, Ying-Jun
三七（Panax notoginseng（Burk.）F.H. Chen（五加科），这是一个著名的传统中药，既在原料和治疗的形式使用了很长一段时间，导致了一个新的达玛烷型皂苷，即三七ST-4的分离。经1D和2D核磁共振谱及酸水解分析，确定其结构为3，12，20（S）-tri-hydroxydammar-24-ene-3-O-d-xylopyranosyl-（12）--d-glucopyranosyl-（12）--d-glu-copyranoside。在体外研究了NotoST-4对单纯疱疹病毒1型（HSV-1）和2型（HSV-2）的抗病毒活性。通过空斑减少试验测定的HSV-1和HSV-2的50%有效浓度（EC 50）值分别为16.47 +/- 0.67和19.44 +/-1.16 M，而通过Vero细胞XTT试验测定的50%细胞毒性浓度（CC 50）为510.64 +/-4.56 M。通过粘附试验和基于空斑减少试验的穿透试验分析，三七素ST-4的抗病毒活性主要是由于穿透抑制作用，这通过荧光显微镜观察证实三七素ST-4阻断病毒的穿透。因此，NotoST-4可能是一种有前途的治疗单纯疱疹病毒感染的药物。
Further study on steam-treated notoginseng, the roots of Panax notoginseng (Burk.) F.H. Chen (Araliaceae), which is a famous traditional Chinese medicine that is used both in raw and treated forms for a long time, led to the isolation of a new dammarane-type saponin, namely notoginsenoside ST-4. Its structure was elucidated to be 3,12,20(S)-tri-hydroxydammar-24-ene-3-O--d-xylopyranosyl-(12)--d-glucopyranosyl-(12)--d-glu-copyranoside, based on the detailed analyses of the 1D and 2D NMR spectral data and acidic hydrolysis. Notoginsenoside ST-4 was investigated for its antiviral activity on herpes simplex type 1 (HSV-1) and type 2 (HSV-2) in vitro. The 50% effective concentration (EC50) values, determined by plaque reduction assay, were 16.47 +/- 0.67 and 19.44 +/- 1.16M for HSV-1 and HSV-2, respectively, whereas the 50% cytotoxic concentration (CC50) determined by the XTT test on Vero cells was 510.64 +/- 4.56M. As analyzed by attachment assay and penetration assay based on plaque reduction assay, the antiviral activity of notoginsenoside ST-4 was principally due to the penetration inhibition effects, which was confirmed by fluorescence microscopy observation that notoginsenoside ST-4 blocked the penetration of virus. Therefore, notoginsenoside ST-4 might be a promising agent for herpes simplex virus infection.