BEHAVIOR OF A CALCIUM-PHOSPHATE CEMENT IN SIMULATED BLOOD-PLASMA IN-VITRO
BEHAVIOR OF A CALCIUM-PHOSPHATE CEMENT IN SIMULATED BLOOD-PLASMA IN-VITRO
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DOI：
10.1016/0109-5641(94)90018-3
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发表时间：
1994-01-01
期刊：
影响因子：
5
通讯作者：
ASAOKA, K
中科院分区：
文献类型：
--
作者：
ISHIKAWA, K;TAKAGI, S;ASAOKA, K
目标。本研究的目的是为了更好地了解磷酸钙骨水泥（CPC）植入物融入生物组织的方法。采用体外连续流动系统来检查这种生物活性材料的盘状样品在持续的类似生理溶液条件下的延长行为。使用重量测量、径向拉伸强度测量 (DTS)、扫描电子显微镜 (SEM)、傅里叶变换红外光谱 (FTIR) 和粉末 X 射线衍射 (XRD) 来表征 CPC 样品随浸泡时间的变化。结果。当CPC浸入Ca（2.5 mmol/L）和无机磷酸盐（1.0 mmol/L）水平恒定的模拟血浆中时，在20周的时间内，样本的重量和DTS稳定地增加至其原始值的约1.5倍。 SEM 观察显示新的沉淀物形成与原始 CPC 表面紧密接触。 FTIR 和 XRD 分析表明，沉淀物是 B 型碳酸盐羟基磷灰石 (OHAp)，这是在骨骼和牙本质中观察到的 OHAp 类型。另一方面，CPC 圆盘内部的堆积密度或 OHAP 含量并未增加。因此，重量和 DTS 的增加归因于 CPC 表面上的 OHAP 沉淀。显着性。结果表明，在体内条件下，CPC 植入物不会溶解在生理液体中。 OHAP 涂层可以在植入物上形成，这可以通过机械强化植入物与骨骼之间的界面来增强植入物与骨骼的结合。
Objectives. The purpose of this study was to gain a better understanding of the integration of calcium phosphate cement (CPC) implants in biological tissueMethods. An in vitro continuous flow system was employed to examine the protracted behavior of disc-shaped specimens of this bioactive material under sustained physiological-like solution conditions. Weight measurement, diametral tensile strength measurement (DTS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and powder x-ray diffraction (XRD) were used to characterize the CPC samples as a function of immersion time.Results. When CPC was immersed in simulated blood plasma in which the Ca (2.5 mmol/L) and inorganic phosphate (1.0 mmol/L) levels were kept constant, both the weight and DTS of the specimens steadily increased to about 1.5 times their original values over a period of 20 wk. SEM observations showed new precipitate formations in intimate contact with the original CPC surface. FTIR and XRD analyses revealed that the precipitate was a B-type carbonate hydroxyapatite (OHAp), the type of OHAp observed in bone and dentin. On the other hand, the interior of CPC discs did not show an increase in either bulk density or OHAp content. Thus, the increases in weight and DTS are attributal to the OHAp precipitation on the CPC surface.Significance. The results suggest that under in vivo conditions, CPC implants would not dissolve in physiological fluids. OHAp coatings may form on the implants, which may enhance bonding of implants to bone by mechanically strengthening the interface between them.