Conversion of adult mouse unipotent germline stem cells into pluripotent stem cells
Conversion of adult mouse unipotent germline stem cells into pluripotent stem cells
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DOI：
10.1038/nprot.2010.44
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发表时间：
2010-01-01
期刊：
影响因子：
14.8
通讯作者：
Schoeler, Hans R.
中科院分区：
文献类型：
--
作者：
Ko, Kinarm;Arauzo-Bravo, Marcos J.;Schoeler, Hans R.
生殖系干细胞(GSCs)，通常被称为精原干细胞，是一种仅能产生配子的单能干细胞。在特定的培养条件下，单能干细胞可以转化为多能干细胞，称为生殖系来源的多能干细胞(GPS)。GPS细胞可以分化为形成所有三个生殖层的细胞和生殖细胞。在这项研究中，我们描述了一种简单而健壮的方法，用于从成年小鼠睾丸中获得GSCs，并将GSCs快速且可重复地转化为GPS细胞。在我们确定的培养条件下，GSCs可以在大约1个月的时间内转化为GPS细胞。GSCs的初始平板数量和培养时间是GSCs成功转化为GPS细胞的两个关键条件。从分子和细胞特性及其发展潜力来看，GPS细胞类似于胚胎干细胞。因此，GPS细胞的产生具有组织再生医学的潜力。
Germline stem cells (GSCs), often called spermatogonial stem cells, are unipotent stem cells that can give rise only to gametes. Under defined culture conditions, unipotent GSCs can be converted into pluripotent stem cells, termed as germline-derived pluripotent stem (gPS) cells. gPS cells can be differentiated into cells forming all three germ layers and germ cells. In this study, we describe a simple and robust protocol for the derivation of GSCs from adult mouse testis and the rapid and reproducible conversion of GSCs into gPS cells. Under our defined culture conditions, GSCs can be converted into gPS cells in similar to 1 month. The initial number of plated GSCs and the culture time are two conditions that are critical for the successful conversion of GSCs into gPS cells. gPS cells are similar to embryonic stem cells, as judged by molecular and cellular properties and its development potential. Thus, generation of gPS cells holds potential for tissue regenerative medicine.