Correlation of hormone receptor status between circulating tumor cells, primary tumor, and metastasis in breast cancer patients.
Correlation of hormone receptor status between circulating tumor cells, primary tumor, and metastasis in breast cancer patients.
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DOI：
10.1007/s12094-015-1275-1
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发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Bischoff FZ
中科院分区：
文献类型：
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作者：
Kalinsky K;Mayer JA;Xu X;Pham T;Wong KL;Villarin E;Pircher TJ;Brown M;Maurer MA;Bischoff FZ
雌激素受体(ER)和孕激素受体(PR)状态对乳腺癌的预后和预测有重要意义。由于转移性乳腺肿瘤活检在常规上是不可行的，循环肿瘤细胞(CTCs)提供了一种确定ER/PR肿瘤状态的替代来源。前瞻性采集36例转移性乳腺癌患者的外周血。用微流控OncoCEE™平台分离CTCs。检测用扩展的抗细胞角蛋白(CK)鸡尾酒混合物和抗CD45完成。免疫细胞化学(ICC)检测CK+细胞ER/PR蛋白的表达，并与原发灶和/或转移瘤(免疫组化：IHC)进行比较。在24例CK+CTC中，原发乳腺癌ER/PR与CTC的符合率为68%(15/22)，转移瘤与CTC的ER/PR符合率为83%(10/12)。在评估CTC和转移性肿瘤(如果没有转移性乳腺活检可替代原发肿瘤)时，总体符合率为79%(19/24)。将CTCs与肿瘤组织进行比较，其敏感性为72%，特异性为100%。在7例CTC与原发灶组织不一致的病例中，2例与转移活检结果一致。用™平台检测肿瘤组织ER/PR状态是可行的，肿瘤组织与肿瘤组织之间ER/PR状态具有较高的一致性。CTC ER/PR蛋白表达的预后和预测意义需要在更大规模的试验中进一步评估。
Estrogen receptor (ER) and progesterone receptor (PR) status is prognostic and predictive in breast cancer. Because metastatic breast tumor biopsies are not routinely feasible, circulating tumor cells (CTCs) offer an alternative source of determining ER/PR tumor status. Peripheral blood was collected prospectively from 36 patients with metastatic breast cancer. CTCs were isolated using the microfluidic OncoCEE™ platform. Detection was accomplished with an expanded anti-cytokeratin (CK) cocktail mixture and anti-CD45. ER/PR protein expression was assessed by immunocytochemistry (ICC) on the CK+ cells and compared to the primary and/or metastatic tumor (immunohistochemistry: IHC). Among the 24 CK+ CTC cases, a concordance of 68% (15/22) in ER/PR status between primary breast tumor and CTCs and 83% (10/12) between metastatic tumor and CTCs was observed. An overall concordance of 79% (19/24) was achieved when assessing CTC and metastatic tumor (primary tumor substituted if metastatic breast biopsy not available). A test sensitivity of 72% and specificity of 100% was identified when comparing CTCs to tumor tissue. Of the 7 discordant cases between CTCs and primary tumor tissue, 2 were concordant with the metastatic biopsy. CTC ER/PR status using the OncoCEE™ platform is feasible, with high concordance in ER/PR status between tumor tissue (IHC) and CTCs (ICC). The prognostic and predictive significance of CTC ER/PR protein expression needs further evaluation in larger trials.