Biochemical characterization of a chromosomal toxin–antitoxin system in Mycobacterium tuberculosis
Biochemical characterization of a chromosomal toxin–antitoxin system in Mycobacterium tuberculosis
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DOI：
10.1016/j.febslet.2008.01.045
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发表时间：
2008-03
期刊：
影响因子：
3.5
通讯作者：
Longxuan Zhao;Junjie Zhang
中科院分区：
文献类型：
--
作者：
Longxuan Zhao;Junjie Zhang
在本文中，我们报告了结核分枝杆菌的染色体毒素-抗毒素（TA）系统的生化特性，包括Rv 1991 c基因和它的上游开放阅读框（ORF）称为Rv 1991 a。Rv 1991 c是一种具有核糖核酸酶活性的毒素，Rv 1991 a是Rv 1991 c的抗毒素。Rv 1991 a与Rv 1991 c相互作用形成复合物。一个启动子位于Rv 1991 a的上游。Rv 1991 a和Rv 1991 a-Rv 1991 c复合物都能够与Rv 1991 a-Rv 1991 c操纵子的启动子区结合，表明Rv 1991 a-Rv 1991 c操纵子的表达可以被自动调节。
In the present paper, we report the biochemical characterization of a chromosomal toxin–antitoxin (TA) system in Mycobacterium tuberculosis, consisting of the Rv1991c gene and its upstream open reading frame (ORF) termed Rv1991a. Rv1991c was characterized as a toxin with ribonuclease activity and Rv1991a as the antitoxin against Rv1991c. Rv1991a interacted with Rv1991c to form a complex. A promoter located immediately upstream of Rv1991a was identified. Both Rv1991a and the Rv1991a–Rv1991c complex were able to bind to the promoter region of the Rv1991a–Rv1991c operon, indicating that the expression of the Rv1991a–Rv1991c operon can be autoregulated.