Viability and electrophysiology of neural cell structures generated by the inkjet printing method
Viability and electrophysiology of neural cell structures generated by the inkjet printing method
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DOI：
10.1016/j.biomaterials.2006.01.048
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发表时间：
2006-07-01
期刊：
影响因子：
14
通讯作者：
Boland, T
中科院分区：
文献类型：
--
作者：
Xu, T;Gregory, CA;Boland, T
通过自动和直接喷墨打印原始胚胎海马神经元和皮质神经元，创建了复杂的细胞图案和结构。免疫染色分析和全细胞膜片钳记录显示，胚胎海马神经元和皮质神经元在通过热喷墨喷嘴打印后，保持了基本的细胞属性和功能，包括正常、健康的神经元表型和电生理特征。此外，在这项研究中，开发了一种创建3D细胞结构的新方法：通过交替喷墨打印NT2细胞和纤维蛋白凝胶，将神经细胞薄片彼此层叠(逐层处理)。综上所述，这些结果和发现表明，喷墨打印正在迅速演变为一种数字制造方法，以构建功能神经结构，最终可能在神经组织工程中得到应用。(C)2006年，爱思唯尔有限公司出版。
Complex cellular patterns and structures were created by automated and direct inkjet printing of primary embryonic hippocampal and cortical neurons. Immunostaining analysis and whole-cell patch-clamp recordings showed that embryonic hippocampal and cortical neurons maintained basic cellular properties and functions, including normal, healthy neuronal phenotypes and electrophysiological characteristics, after being printed through thermal inkjet nozzles. In addition, in this study a new method was developed to create 3D cellular structures: sheets of neural cells were layered on each other (layer-by-layer process) by alternate inkjet printing of NT2 cells and fibrin gels. These results and findings, taken together, show that inkjet printing is rapidly evolving into a digital fabrication method to build functional neural structures that may eventually find applications in neural tissue engineering. (c) 2006 Published by Elsevier Ltd.