Performance evaluation of the Sysmex pocH-100iV Diff hematology analyzer for analysis of canine, feline, equine, and bovine blood
Performance evaluation of the Sysmex pocH-100iV Diff hematology analyzer for analysis of canine, feline, equine, and bovine blood
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DOI：
10.1111/j.1939-165x.2011.00372.x
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发表时间：
2011-12-01
影响因子：
1.2
通讯作者：
Lutz, Hans
中科院分区：
文献类型：
--
作者：
Riond, Barbara;Weissenbacher, Stefan;Lutz, Hans
背景资料：Sysmex pocH-100 iV Diff是最近在欧洲兽医实践中引入的用于床旁使用的阻抗血液分析仪。目的：本研究的目的是验证pocH-100 iV Diff用于分析狗、猫、马和牛的血液样本。方法：在pocH-100 iV Diff和Cell-Dyn 3500上分析了来自健康和患病犬（115）、猫（94）、马（91）和牛（78）的新鲜EDTA血样。对77只犬、65只猫、40匹马和46头牛的自动WBC分类计数结果与手动分类计数结果进行了比较。将HCT与通过微量红细胞压积离心获得的PCV进行比较。此外，精密度，线性，携带，细胞老化，和临床相关性的pocH-100 iV Diff results.Results：大多数的CBC结果得到的pocH-100 iV Diff相关以及与细胞-动态3500。观察到犬MCV和血红蛋白浓度的相关性略低。马和牛的淋巴细胞相关性很好，但猫和狗的相关性较差。被称为“OTHRS”的混合细胞群（马和牛的所有粒细胞和单核细胞;猫和犬的中性粒细胞、单核细胞和嗜碱性粒细胞）在所有供试种属中相关性良好。该仪器高估了猫和犬的嗜酸性粒细胞。在猫中，血小板计数显示出很强的负偏差。结论：pocH-100 iV Diff的整体性能是优秀的注意到的局限性。自动分类计数可用作筛查工具，与血液涂片评估结合使用。
Background: The Sysmex pocH-100iV Diff is an impedance hematology analyzer recently introduced for point-of-care use in veterinary practices in Europe.Objective: The purpose of this study was to validate the pocH-100iV Diff for analysis of blood samples from dogs, cats, horses, and cattle.Methods: Fresh EDTA-blood samples from healthy and ill dogs (115), cats (94), horses (91), and cattle (78) were analyzed on the pocH-100iV Diff and the Cell-Dyn 3500. Results of the automated WBC differential counts were compared with the manual differential counts for 77 dogs, 65 cats, 40 horses, and 46 cattle. HCT were compared with PCVs obtained by microhematocrit centrifugation. Furthermore, precision, linearity, carry-over, cell aging, and clinical relevance of the pocH-100iV Diff results were assessed.Results: Most of the CBC results obtained by the pocH-100iV Diff correlated well with those of the Cell-Dyn 3500. Slightly low correlation was observed for canine MCV and hemoglobin concentration. Lymphocytes correlated well in horses and cattle, but less well in cats and dogs. The mixed cell population termed "OTHRS" (all granulocytes and monocytes for horses and cattle; neutrophils, monocytes, and basophils for cats and dogs) correlated well in all tested species. The instrument overestimated feline and canine eosinophils. In cats, platelet counts showed a strong negative bias.Conclusions: The overall performance of the pocH-100iV Diff was excellent with the noted limitations. The automated differential count can be used as screening tool in conjunction with evaluation of a blood smear.