An MHC-restricted CD8+ T-cell response is induced in cattle by foot-and-mouth disease virus (FMDV) infection and also following vaccination with inactivated FMDV
An MHC-restricted CD8+ T-cell response is induced in cattle by foot-and-mouth disease virus (FMDV) infection and also following vaccination with inactivated FMDV
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DOI：
10.1099/vir.0.83417-0
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发表时间：
2008-03-01
影响因子：
3.8
通讯作者：
Ellis, Shirley A.
中科院分区：
文献类型：
--
作者：
Guzman, Efrain;Taylor, Geraldine;Ellis, Shirley A.
口蹄疫病毒（FMDV）引起偶蹄动物的高度传染性疾病，带来巨大的经济后果。CD8（+）细胞毒性T淋巴细胞在病毒感染的保护和疾病结局中起重要作用，但迄今为止，CD8（+）T细胞对FMDV的免疫应答的作用仍不清楚。本研究旨在研究免疫牛和感染牛对口蹄疫病毒的主要组织相容性复合体（MHC）I类限制性CD8（+）T细胞应答。体外试验用于检测已知MHC I类基因型的口蹄疫病毒感染牛中CD8（+）T细胞释放的抗原特异性γ干扰素。一个显着的MHC I类限制性CD8+ T细胞反应检测到FMDV O1株BFS和重组鸡痘病毒表达FMDV的结构蛋白。抗原特异性MHC I类限制性CD8+ T细胞反应也检测到牛接种灭活口蹄疫病毒。这些反应被证明是直接的，至少部分，在病毒的结构蛋白（P12A区）内的表位。通过使用表达单一牛MHC I类等位基因的小鼠细胞，有可能鉴定每种情况下的限制性元件。这些表位的鉴定将有助于对牛中口蹄疫病毒特异性记忆性CD8（+）T细胞进行定量和定性分析，并有助于确保潜在的疫苗诱导定性适当的CD8（+）T细胞应答。
Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hooved animals that carries enormous economic consequences. CD8(+) cytotoxic T lymphocytes play an important role in protection and disease outcome in viral infections but, to date, the role of the CD8(+) T-cell immune response to FMDV remains unclear. This study aimed to investigate major histocompatibility complex (MHC) class I-restricted CD8(+) T-cell responses to FMDV in vaccinated and in infected cattle. An in vitro assay was used to detect antigen-specific gamma interferon release by CD8(+) T cells in FMDV-infected cattle of known MHC class I genotypes. A significant MHC class I-restricted CD8+ T-cell response was detected to both FMDV strain O1 BFS and a recombinant fowlpox virus expressing the structural proteins of FMDV. Antigen-specific MHC class I-restricted CD8+ T-cell responses were also detected in cattle vaccinated with inactivated FMDV. These responses were shown to be directed, at least in part, to epitopes within the structural proteins (P12A region) of the virus. By using mouse cells expressing single cattle MHC class I alleles, it was possible to identify the restriction elements in each case. Identification of these epitopes will facilitate the quantitative and qualitative analysis of FMDV-specific memory CD8(+) T cells in cattle and help to ensure that potential vaccines induce a qualitatively appropriate CD8(+) T-cell response.