Calmodulin7 plays an important role as transcriptional regulator in Arabidopsis seedling development
Calmodulin7 plays an important role as transcriptional regulator in Arabidopsis seedling development
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DOI：
10.1105/tpc.107.057612
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发表时间：
2008-07-01
期刊：
影响因子：
11.6
通讯作者：
Chattopadhyay, Sudip
中科院分区：
文献类型：
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作者：
Kushwaha, Ritu;Singh, Aparna;Chattopadhyay, Sudip
钙调素（CaM）在真核生物中具有多种调节作用，但其作为转录调节因子的直接功能尚不清楚。此外，CaM在植物中的生理功能在很大程度上是未知的。在这里，我们表明，四个拟南芥钙调素亚型之一，CAM 7，是一个转录调节，直接与光诱导基因的启动子相互作用，促进光形态建成。CAM7过表达导致超光形态发生生长和光诱导基因表达的增加。CAM 7突变对光形态发生生长没有明显影响，表明可能存在冗余基因功能。然而，cam7突变体显示减少的光诱导基因的表达，cam7 hy5双突变体显示hy5表型的增强。此外，CAM 7的过表达可以部分抑制hy5表型，表明这两个因素共同作用，以控制光诱导的幼苗发育。突变和转基因研究，以及生理分析，说明CAM 7和HY5碱性亮氨酸拉链转录因子在拟南芥幼苗发育中的协同作用。
Although calmodulin (CaM) is known to play multiple regulatory roles in eukaryotes, its direct function as transcriptional regulator is unknown. Furthermore, the physiological functions of CaM are largely unknown in plants. Here, we show that one of the four Arabidopsis thaliana CaM isoforms, CAM7, is a transcriptional regulator that directly interacts with the promoters of light-inducible genes and promotes photomorphogenesis. CAM7 overexpression causes hyperphotomorphogenic growth and an increase in the expression of light-inducible genes. Mutations in CAM7 produce no visible effects on photomorphogenic growth, indicating likely redundant gene functions. However, cam7 mutants display reduced expression of light-inducible genes, and cam7 hy5 double mutants show an enhancement of the hy5 phenotype. Moreover, overexpression of CAM7 can partly suppress the hy5 phenotype, indicating that the two factors work together to control light-induced seedling development. The mutational and transgenic studies, together with physiological analyses, illustrate the concerted function of CAM7 and HY5 basic leucine zipper transcription factor in Arabidopsis seedling development.