Mapping of quantitative trait loci associated with ultraviolet-B resistance in rice (Oryza sativa L.)
Mapping of quantitative trait loci associated with ultraviolet-B resistance in rice (Oryza sativa L.)
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DOI：
10.1007/s00122-003-1353-6
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发表时间：
2003-07
影响因子：
5.4
通讯作者：
Tadashi Sato;T. Ueda;Y. Fukuta;Tadashi Kumagai;Masahiro Yano
中科院分区：
文献类型：
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作者：
Tadashi Sato;T. Ueda;Y. Fukuta;Tadashi Kumagai;Masahiro Yano
水稻抗UV-B数量性状基因座（QTL）的检测将有助于其在抗UV-B育种中的实际应用，并可能导致基因特性和功能的鉴定。栽培稻（Oryza sativa L.）的UV-B抗性存在相当大的变异，但其具体的遗传调控机制尚未完全阐明。利用98个来自日本晴（一个抗UV-B辐射的品种）和Kasalath（一个敏感的籼稻品种）的BC 1F 5（回交自交系; BILs）检测了水稻抗UV-B辐射增强的QTL。我们使用245个RFLP标记构建了一个框架连锁图。BIL和两个亲本在生长室中在可见光下生长，有或没有补充UV-B辐射。为了评估UV-B抗性，我们使用了地上部分的相对鲜重（RFW）和叶片的相对叶绿素含量（RCC）。BIL群体在RFW和RCC中表现出广泛的变化。利用LOD阈值为2.9的复合区间作图，在第1、3和10染色体上检测到3个与RFW和RCC相关的QTL。位于第1和10染色体QTL上的日本晴等位基因增加了RFW和RCC，而位于第3染色体QTL上的Kasalath等位基因增加了这两个性状。此外，利用染色体片段代换系证实了在第1和第10染色体上存在两个抗UV-B的QTL。在第10染色体上的QTL的Kasalath等位基因的植物更敏感的UV-B辐射比植物与他们在第1染色体上。这些结果不仅为利用分子标记联合选择改良水稻的抗UV-B能力提供了依据，而且也为利用近等基因系研究水稻的抗UV-B机制提供了依据。
The detection of quantitative trait loci (QTLs) associated with UV-B resistance in rice should allow their practical application in breeding for such a complex trait, and may lead to the identification of gene characteristics and functions. Considerable variation in UV-B resistance exists within cultivated rice (Oryza sativaL.), but its detailed genetic control mechanism has not been well elucidated. We detected putative QTLs associated with the resistance to enhanced UV-B radiation in rice, using 98 BC1F5(backcross inbred lines; BILs) derived from a cross between Nipponbare (a resistantjaponicarice variety) and Kasalath (a sensitiveindicarice variety). We used 245 RFLP markers to construct a framework linkage map. BILs and both parents were grown under visible light with or without supplemental UV-B radiation in a growth chamber. In order to evaluate UV-B resistance, we used the relative fresh weight of aerial parts (RFW) and the relative chlorophyll content of leaf blades (RCC). The BIL population exhibited a wide range of variation in RFW and RCC. Using composite interval mapping with a LOD threshold of 2.9, three putative QTLs associated with both RFW and RCC were detected on chromosomes 1, 3 and 10. Nipponbare alleles at the QTLs on chromosome 1 and 10 increased the RFW and RCC, while the Kasalath allele at the QTL on chromosome 3 increased both traits. Furthermore, the existence of both QTLs on chromosomes 1 and 10 for UV-B resistance was confirmed using chromosome segment substitution lines. Plants with Kasalath alleles at the QTL on chromosome 10 were more sensitive to UV-B radiation than plants with them on chromosome 1. These results also provide the information not only for the improvement of UV-B resistance in rice though marker-associated selection, but also for the identification of UV-B resistance mechanisms by using near-isogenic lines.