FIBRONECTIN INVOLVEMENT IN GRANULATION-TISSUE AND WOUND-HEALING IN RABBITS
FIBRONECTIN INVOLVEMENT IN GRANULATION-TISSUE AND WOUND-HEALING IN RABBITS
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DOI：
10.1177/30.4.6174568
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发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
FURCHT, LT
中科院分区：
文献类型：
--
作者：
REPESH, LA;FITZGERALD, TJ;FURCHT, LT
本研究描述了纤维连接蛋白在兔浅层创面中的分布及其与网状纤维(III型胶原)和透明质酸的关系。在背部用外科刀片切开线形切口。分别于伤后1、2、3、4、5、8天处死动物，用过氧化物酶-抗过氧化物酶标记亲和纯化的纤维连接蛋白抗体。除了网状蛋白的银染和透明质酸的阿尔新蓝染色外，组织样本还被苏木精和曙红染色。创伤后，伤口充满纤维蛋白凝块，纤维连接蛋白染色呈阳性。下面的真皮和邻近的未受伤真皮也含有纤维连接蛋白。从伤口边缘迁移到血块和真皮之间的表皮细胞与这些伤口成分中的纤维连接蛋白直接相关。72小时后，表皮的连续性得以重建。早期肉芽组织在第5天创面的表皮下可见。纤维连接蛋白在单个成纤维细胞周围的基质中可见，并与网状纤维和透明质酸共存于第5天和第8天创面。8天创面肉芽组织纤维粘连蛋白染色较深，延伸至真皮网状层。纤维连接蛋白和成纤维细胞的致密纤维网络平行于表皮排列，使肉芽组织具有高度结构化和组织化的外观。成纤维细胞含有纤维连接蛋白，伤口周围的纤维连接蛋白比肉芽组织内的少。纤维连接蛋白可能在修复过程中作为迁移细胞的细胞外支架而在组织重建中发挥重要作用。
This study describes the distribution of fibronectin and its association with reticulin fibers (type III collagen) and hyaluronic acid in shallow rabbit wounds. Linear incisions were made dorsally with a surgical blade. Animals were sacrificed and 1, 2, 3, 4, 5 and 8 day wounds were examined using peroxidase-antiperoxidase to localize affinity-purified antibodies to fibronectin. Tissue samples were also stained with hematoxylin and eosin in addition to silver stains for reticulin and Alcian blue for hyaluronic acid. After wounding, the incision filled with a fibrin clot that stained positively for fibronectin. The underlying dermis and adjacent, unwounded dermis also contained fibronectin. Epidermal cells that migrated from the wound margin between the clot and the dermis were in direct association with fibronectin in these wound components. By 72 h, epidermal continuity was reestablished. Early granulation tissue formation was apparent just below the epidermis in 5 day wounds. Fibronectin was observed in the matrix surrounding individual fibroblasts and codistributed with reticulin fibers and hyaluronic acid in both 5 and 8 day wounds. Granulation tissue of 8 day wounds stained intensely for fibronectin and extended to a greater depth in the reticular dermis. Dense fibrillar networks of fibronectin and fibroblasts were aligned parallel to the epidermis, giving the granulation tissue a highly structured and organized appearance. Fibroblasts contained fibronectin and were surrounded by less fibronectin at the wound periphery than within the granulation tissue. Fibronectin may be important in the reconstruction of tissues during repair by functioning as an extracellular scaffold for migrating cells.