Instructions for use Title Identification and expression analysis of the genes involved in serotonin biosynthesis and transduction in the field cricket
Instructions for use Title Identification and expression analysis of the genes involved in serotonin biosynthesis and transduction in the field cricket
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发表时间：
2017
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通讯作者：
Takayuki Watanabe;H. Sadamoto;H. Aonuma
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作者：
Takayuki Watanabe;H. Sadamoto;H. Aonuma
5-羟色胺(5-HT)对板球的攻击性行为和昼夜节律行为等行为起着多方面的调节作用。为了阐明板球5-羟色胺系统的分子基础，我们在野外板球动物Gryllus bimaculatus DeGeer中克隆了5-HT相关基因。从蟋蟀脑文库中分离到了色氨酸转化为5-羟基-L-色氨酸(5-HTP)的色氨酸羟基酶和苯丙氨酸-色氨酸羟基酶(TPH)以及芳香族L氨基酸脱羧酶(AADC)的互补基因。此外，还鉴定了4个5-羟色胺受体基因(5-HT1a、5-HT1B、5HT2α和5-HT7)。对选择性参与果蝇神经元和外周5-羟色胺合成的TRH和TPH的表达分析表明，蟋蟀神经组织中同时存在两条5-羟色胺合成途径。其中4个5-羟色胺受体基因在不同组织中表达水平不同，提示5-羟色胺系统在蟋蟀体内广泛分布。
Serotonin (5-HT) modulates various aspects of behaviors such as aggressive behavior and circadian behavior in the cricket. To elucidate the molecular basis of the cricket 5-HT system, we identified 5-HT-related genes in the field cricket Gryllus bimaculatus DeGeer. Complementary DNA of tryptophan hydroxylase (TRH) and phenylalanine-tryptophan hydroxylase (TPH) which convert tryptophan into 5-hydroxy-L-tryptophan (5-HTP), and that of aromatic L-amino acid decarboxylase (AADC) which converts 5-HTP into 5-HT were isolated from a cricket brain cDNA library. In addition, four 5-HT receptor genes (5-HT1A, 5-HT1B, 5HT2α, and 5-HT7) were identified. Expression analysis of TRH and TPH, which are selectively involved in neuronal and peripheral 5-HT synthesis in Drosophila, suggested that two 5-HT synthesis pathways co-exist in the cricket neuronal tissues. The four of 5-HT receptor genes were expressed in various tissues at differential expression levels, suggesting that the 5-HT system is widely distributed in the cricket.