Tracking Single Molecular Diffusion on Glass Substrate Modified with Periodic Ag Nano-architecture
Tracking Single Molecular Diffusion on Glass Substrate Modified with Periodic Ag Nano-architecture
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DOI：
10.1143/jjap.45.6039
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发表时间：
2006-07
影响因子：
1.5
通讯作者：
B. Takimoto;H. Nabika;K. Murakoshi
中科院分区：
文献类型：
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作者：
B. Takimoto;H. Nabika;K. Murakoshi
利用全内反射荧光显微镜（TIRFM）直接观察了水溶液中单个染料标记分子在玻璃基底上自扩散脂质双层中的扩散情况。通过对单个分子轨迹的均方位移（MSD）分析对分子扩散进行了定量评估。在基底表面存在周期性三角形银纳米粒子时，脂质双层中正常的布朗扩散模式发生了改变。在含有三角形银纳米粒子的体系中观察到的扩散率降低归因于分子运动被限制在银三角形所包围的微小区域内。
Diffusion of individual dye-labeled molecules in self-spreading lipid bilayer on glass substrate in aqueous solution was observed directly using total internal reflection fluorescence microscopy (TIRFM). The molecular diffusion was evaluated quantitatively by the mean square displacement (MSD) analysis for the trajectories of individual molecules. The mode of normal Brownian diffusion in lipid bilayer was changed in the presence of the periodic triangular Ag nanoparticles on the surface of the substrate. Suppressed diffusivity observed at the system with the triangular Ag nanoparticles was attributed to the confinement of the molecular motion in the micro-compartment surrounded by the Ag triangles.