Creatine kinase, myokinase, and acetylcholinesterase activities in muscle-forming primary cultures of mouse teratocarcinoma cells
Creatine kinase, myokinase, and acetylcholinesterase activities in muscle-forming primary cultures of mouse teratocarcinoma cells
复制标题
小鼠畸胎癌细胞肌肉形成原代培养物中的肌酸激酶、肌激酶和乙酰胆碱酯酶活性
DOI：
10.1016/0092-8674(75)90074-4
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发表时间：
1975
期刊：
影响因子：
64.5
通讯作者：
B. Mintz
中科院分区：
文献类型：
--
作者：
J. Gearhart;B. Mintz
将多能小鼠畸胎瘤细胞胚状体接种于塑料或胶原基质上。细胞决定的各种模式，包括生肌细胞，发生。分化的主要途径很快成为肌原细胞：许多多核肌管形成并产生了骨骼肌纤维的延伸网络。这个过程没有进行到正常的完成，因为纤维缺乏条纹并且不能收缩。检测了几种通常与肌肉分化有关的酶的活性。乙酰胆碱酯酶活性增加，特别是在肌管形成期间，如在正常的肌发生中。然而，肌酸激酶活性在肌管形成期间升高，然后异常下降，并且肌激酶活性未能明显增加。肌酸激酶的胎儿同工酶形式在培养物中表达，尽管如果骨骼肌明显存在，则从小鼠中取出的分化良好的实体瘤显示达到成人肌肉同工酶类型。结果是一致的解释，协调调控的基因表达的变化控制这些功能可能需要后期阶段的肌肉发生。
Multipotential mouse teratocarcinoma ceils in embryoid bodies were explanted on plastic or collagen substrates. Varlous modes of cell determination, including myogenesls, occurred. The predominant avenue of dlfferentlation soon became myogenesls: many multlnucleated myotubes formed and yielded an extenslve network of skeletal muscle fibers. The process does not proceed to normal completion, as the flbers have a paucity of striations and are not contractile. Activltles of several enzymes ordinarily associated with muscle differentiation were examined. Acetylcholinesterase activity Increases, especially during myotube formation, as In normal myogenesis. However, creatine kinase activity rises durlng myotube formatlon and then drops abnormally, and myokinase activity fails to increase appreciably. The fetal isozymlc form of creatine kinase is expressed in the cultures, although well differentiated solid tumors taken from mice show attainment of the adult muscle isozyme type if skeletal muscle is demonstrably present. The results are consistent with the interpretation that coordinately regulated changes in gene expressions controlling these functions may be required for later stages of myogenesis.