Slit and glypican-1 mRNAs are coexpressed in the reactive astrocytes of the injured adult brain
Slit and glypican-1 mRNAs are coexpressed in the reactive astrocytes of the injured adult brain
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作者：
Hagino, S;Iseki, K;Wanaka, A
在神经发育过程中，狭缝家族作为一种排斥剂，用于使轴突朝着正确的目标生长。最近的一份报告描述了成年大鼠不同脑区表达的狭缝mRNA。然而，它们在成人神经系统中的功能仍然未知。在本研究中，我们调查是否狭缝mRNA表达在冷冻损伤的大脑，使用原位杂交。所有狭缝家族成员均在病变处表达。Slit 2 mRNA在坏死组织周围的细胞中表达最强。双标记研究显示，slit 2 mRNA在胶质细胞酸性蛋白（GFAP）阳性反应性星形胶质细胞中表达。此外，磷脂酰肌醇蛋白聚糖-1，硫酸乙酰肝素蛋白聚糖，作为一个高亲和力受体的Slit蛋白，与slit 2 mRNA在反应性星形胶质细胞共表达。这些发现表明，slit 2可能会阻止再生轴突进入病变与磷脂酰肌醇蛋白聚糖-1。
The slit family serves as a repellent for growing axons toward correct targets during neural development. A recent report describes slit mRNAs expressed in various brain regions in adult rats. However, their functions in the adult nervous system remain unknown. In the present study, we investigated whether slit mRNAs were expressed in the cryo-injured brain, using in situ hybridization. All slit family members were expressed at the lesion. Slit2 mRNA was the most intensely expressed in the cells surrounding the necrotic tissue. A double-labeling study showed that slit2 mRNA was expressed in the glial fibrillary acidic protein (GFAP)-positive reactive astrocytes. In addition, glypican-1, a heparan sulfate proteoglycan that serves as a high-affinity receptor for Slit protein, was coexpressed with slit2 mRNA in the reactive astrocytes. These findings suggested that slit2 might prevent regenerating axons from entering into the lesion in concert with glypican-1.