Rapid Detection of Human Immunodeficiency Virus Types 1 and 2 by Use of an Improved Piezoelectric Biosensor
Rapid Detection of Human Immunodeficiency Virus Types 1 and 2 by Use of an Improved Piezoelectric Biosensor
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DOI：
10.1128/jcm.03041-12
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发表时间：
2013-06-01
影响因子：
9.4
通讯作者：
Larson, Richard S.
中科院分区：
文献类型：
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作者：
Bisoffi, Marco;Severns, Virginia;Larson, Richard S.
灾难可以创造献血可以挽救生命的情况。然而，在紧急情况下和资源耗尽时，现场献血需要迅速和准确地检测血液传播的病原体，包括1型和2型人体免疫缺陷病毒（HIV-1和HIV-2）。PCR和通过酶联免疫吸附测定法（ELISA）捕获HIV-1和HIV-2抗体等技术精确但耗时，需要复杂的设备，不符合紧急护理点的要求。在这里，我们描述了一个原型生物传感器的基础上功能化的压电材料与针对HIV-1和HIV-2的特异性抗体。我们展示了在简单和复杂的解决方案中，包括人血清，以及在存在交叉混杂病毒的情况下，对HIV-1和HIV-2的快速准确检测。我们报告的检测限为12 - 50%的组织培养感染剂量（TCID（50））的HIV-1和87 TCID（50）的HIV-2。与核酸扩增相比，原型生物传感器的准确度、测量精度和操作有利。我们的结论是，生物传感器具有显着的承诺，作为一个成功的床旁诊断设备，用于紧急现场应用，需要快速和可靠的检测血液传播的病原体。
Disasters can create situations in which blood donations can save lives. However, in emergency situations and when resources are depleted, on-site blood donations require the rapid and accurate detection of blood-borne pathogens, including human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2). Techniques such as PCR and antibody capture by an enzyme-linked immunosorbent assay (ELISA) for HIV-1 and HIV-2 are precise but time-consuming and require sophisticated equipment that is not compatible with emergency point-of-care requirements. We describe here a prototype biosensor based on piezoelectric materials functionalized with specific antibodies against HIV-1 and HIV-2. We show the rapid and accurate detection of HIV-1 and HIV-2 in both simple and complex solutions, including human serum, and in the presence of a cross-confounding virus. We report detection limits of 12 50% tissue culture infective doses (TCID(50)s) for HIV-1 and 87 TCID(50)s for HIV-2. The accuracy, precision of measurements, and operation of the prototype biosensor compared favorably to those for nucleic acid amplification. We conclude that the biosensor has significant promise as a successful point-of-care diagnostic device for use in emergency field applications requiring rapid and reliable testing for blood-borne pathogens.