The novel zinc finger-containing transcription factor Osterix is required for osteoblast differentiation and bone formation
The novel zinc finger-containing transcription factor Osterix is required for osteoblast differentiation and bone formation
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DOI：
10.1016/s0092-8674(01)00622-5
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发表时间：
2002-01-11
期刊：
影响因子：
64.5
通讯作者：
de Crombrugghe, B
中科院分区：
文献类型：
--
作者：
Nakashima, K;Zhou, X;de Crombrugghe, B
我们已经确定了一种新的含锌指的转录因子，称为Osterix（Osx），它在所有发育中的骨骼中特异性表达。在Osx缺失小鼠中，没有骨形成发生。在Osx基因缺失小鼠的软骨内骨骼元素中，间充质细胞与破骨细胞和血管一起侵入矿化的软骨基质。然而，间充质细胞不存款骨基质。同样，骨膜和膜性骨骼成分的浓缩间充质中的细胞不能分化成成骨细胞。然而，这些细胞确实表达Runx 2/Cbfa 1，这是骨形成所需的另一种转录因子。相反，Osx在Runx 2/Cbfa 1敲除小鼠中不表达。因此，Osx作用于Runx 2/Cbfa 1的下游。由于Osx空前成骨细胞表达典型的软骨细胞标记基因，我们建议Runx 2/Cbfa 1表达前成骨细胞仍然是双能细胞。
We have identified a novel zinc finger-containing transcription factor, called Osterix (Osx), that is specifically expressed in all developing bones. In Osx null mice, no bone formation occurs. In endochondral skeletal elements of Osx null mice, mesenchymal cells, together with osteoclasts and blood vessels, invade the mineralized cartilage matrix. However, the mesenchymal cells do not deposit bone matrix. Similarly, cells in the periosteum and in the condensed mesenchyme of membranous skeletal elements cannot differentiate into osteoblasts. These cells do, however, express Runx2/Cbfa1, another transcription factor required for bone formation. In contrast, Osx is not expressed in Runx2/Cbfa1 null mice. Thus, Osx acts downstream of Runx2/Cbfa1. Because Osx null preosteoblasts express typical chondrocyte marker genes, we propose that Runx2/Cbfa1-expressing preosteoblasts are still bipotential cells.