Optimization of lycopene extraction from tomato cell suspension culture by response surface methodology.
Optimization of lycopene extraction from tomato cell suspension culture by response surface methodology.
复制标题
DOI：
10.1021/jf801029k
复制
发表时间：
2008-09-10
影响因子：
6.1
通讯作者：
Erdman, John W., Jr.
中科院分区：
文献类型：
--
作者：
Lu, Chi-Hua;Engelmann, Nancy J.;Lila, Mary Ann;Erdman, John W., Jr.
放射性同位素标记的番茄红素是生物医学研究的重要工具，但目前还没有商业化。本实验室已建立了一套生产放射性同位素标记番茄红素的番茄细胞悬浮培养系统。在本研究中，目标是优化番茄细胞培养物中番茄红素的提取效率，用于制备高效液相色谱（HPLC）分离。我们采用响应面法（RSM），它结合了部分析因设计和二次多项式模型。番茄细胞经乙醇匀浆、KOH皂化、正己烷提取后，用HPLC-PDA分析番茄红素的含量。我们在五个水平下改变五个因素：乙醇体积（1.33-4 mL/g）;均质化时间（0-40 s/g）;饱和KOH溶液体积（0-0.67 mL/g）;己烷体积（1.67-3 mL/g）;和涡旋时间（5-25 s/g）。SAS的岭分析表明，提取1g番茄细胞的最佳提取工艺为乙醇1.56 mL、均质28 s、KOH 0.29 mL、己烷2.49 mL、涡旋17.5 s。这些最佳条件下预测的响应面被证实，以提高番茄红素产量从标准化番茄细胞培养超过3倍。
Radioisotope-labeled lycopene is an important tool for biomedical research but currently is not commercially available. A tomato cell suspension culture system for the production of radioisotope-labeled lycopene was previously developed in our laboratory. In the current study, the goal was to optimize the lycopene extraction efficiency from tomato cell cultures for preparatory high-performance liquid chromatography (HPLC) separation. We employed response surface methodology (RSM), which combines fractional factorial design and a second-degree polynomial model. Tomato cells were homogenized with ethanol, saponified by KOH, and extracted with hexane, and the lycopene content was analyzed by HPLC-PDA. We varied five factors at five levels: ethanol volume (1.33–4 mL/g); homogenization period (0–40 s/g); saturated KOH solution volume (0–0.67 mL/g); hexane volume (1.67–3 mL/g); and vortex period (5–25 s/g). Ridge analysis by SAS suggested that the optimal extraction procedure to extract 1 g of tomato cells was at 1.56 mL of ethanol, 28 s homogenization, 0.29 mL of KOH, 2.49 mL of hexane, and 17.5 s vortex. These optimal conditions predicted by RSM were confirmed to enhance lycopene yield from standardized tomato cell cultures by more than 3-fold.
登录
查看更多内容
影响因子：
6.1
作者：
Periago, Maria Jesus;Rincon, Francisco;Ros, Gaspar
通讯作者：
Ros, Gaspar
影响因子：
8.8
作者：
Kaur, Devinder;Wani, Ali Abas;Sogi, D. S.
通讯作者：
Sogi, D. S.
影响因子：
4.2
作者：
Canene-Adams, K;Campbell, JK;Erdman, JW
通讯作者：
Erdman, JW
DOI：
10.1093/jnci/87.23.1767
发表时间：
1995-12-06
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
GIOVANNUCCI, E;ASCHERIO, A;WILLETT, WC
通讯作者：
WILLETT, WC
影响因子：
6.1
作者：
Periago, MJ;Rincón, F;Ros, G
通讯作者：
Ros, G