Mutations in genes controlling the biosynthesis and accumulation of inositol phosphates in seeds
Mutations in genes controlling the biosynthesis and accumulation of inositol phosphates in seeds
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DOI：
10.1042/bst0380689
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发表时间：
2010-04-01
影响因子：
3.9
通讯作者：
Torp, Anna Maria
中科院分区：
文献类型：
--
作者：
Rasmussen, Soren K.;Ingvardsen, Christina Ronn;Torp, Anna Maria
休眠种子中的大部分磷以PA（植酸; InsP（6））的形式储存在蛋白质储存液泡中。PA的生物合成和积累可以从开花后几天开始检测到，并且似乎在种子发育期间持续到成熟。PA生物合成的第一步是通过葡萄糖6-磷酸的转化形成Ins 3 P。随后，肌醇环的其余五个位置被许多激酶连续有序地磷酸化，产生PA。在谷类、豆类和拟南芥中鉴定低PA突变体有助于解析PA的生物合成途径和鉴定控制PA积累的基因。利用诱变和插入元件，在5种植物中鉴定和鉴定了参与PA代谢的7个基因的突变。了解PA在植物种子中的生物合成途径和积累的基因以及PA如何平衡游离磷对于作物特别是谷物和豆类的分子育种具有重要意义。
Most of the phosphorus in the resting seed is stored inside protein storage vacuoles as PA (phytic acid; InsP(6)). The biosynthesis and accumulation of PA can be detected beginning from a few days after anthesis and seem to continue during seed development until maturation. The first step in PA biosynthesis is the formation of Ins3P by conversion of glucose 6-phosphate. This is then followed by a sequential and ordered phosphorylation of the remaining five positions of the inositol ring by a number of kinases, resulting in PA. Identification of low-PA mutants in cereals, legumes and Arabtdopsis is instrumental for resolving the biosynthetic pathway and identification of genes controlling the accumulation of PA. Mutations in seven genes involved in the metabolism of PA have been identified and characterized among five plant species using induced mutagenesis and insertion elements. Understanding the biosynthetic pathway and genes controlling the accumulation of PA in plant seeds and how PA may balance the free phosphate is of importance for molecular breeding of crop plants, particularly cereals and legumes.