The peripheral nervous system supports blood cell homing and survival in the Drosophila larva
The peripheral nervous system supports blood cell homing and survival in the Drosophila larva
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DOI：
10.1242/dev.067322
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发表时间：
2011-12-15
期刊：
影响因子：
4.6
通讯作者：
Brueckner, Katja
中科院分区：
文献类型：
--
作者：
Makhijani, Kalpana;Alexander, Brandy;Brueckner, Katja
造血细胞与其微环境的相互作用控制血细胞定植、归巢和造血。在这里，我们介绍了幼虫造血造血的第一个果蝇模型和外周神经系统（PNS）作为造血微环境的作用。果蝇幼虫的造血系统是由胚胎的分化血细胞建立的，这些血细胞定殖于分段重复的表皮-肌肉口袋并在这些位置增殖。重要的是，我们表明，这些居民血细胞紧密共定位与外周神经元，我们表明，幼虫血细胞依赖于PNS作为一个有吸引力的和营养的微环境。缺乏外周神经元亚群的无调（ATO）突变体或遗传消融的幼虫显示出血细胞的进行性凋亡下降和不完全的常驻血细胞模式，而由前神经基因Scute（SC）的异位表达诱导的额外外周神经元将血细胞错误地引导至这些异位位置。这种PNS-造血连接在果蝇中的PNS在脊椎动物的造血和免疫功能中的新兴作用是平行的，并为将来对PNS-造血轴进行系统的遗传解剖提供了基础。
Interactions of hematopoietic cells with their microenvironment control blood cell colonization, homing and hematopoiesis. Here, we introduce larval hematopoiesis as the first Drosophila model for hematopoietic colonization and the role of the peripheral nervous system (PNS) as a microenvironment in hematopoiesis. The Drosophila larval hematopoietic system is founded by differentiated hemocytes of the embryo, which colonize segmentally repeated epidermal-muscular pockets and proliferate in these locations. Importantly, we show that these resident hemocytes tightly colocalize with peripheral neurons and we demonstrate that larval hemocytes depend on the PNS as an attractive and trophic microenvironment. atonal (ato) mutant or genetically ablated larvae, which are deficient for subsets of peripheral neurons, show a progressive apoptotic decline in hemocytes and an incomplete resident hemocyte pattern, whereas supernumerary peripheral neurons induced by ectopic expression of the proneural gene scute (sc) misdirect hemocytes to these ectopic locations. This PNS-hematopoietic connection in Drosophila parallels the emerging role of the PNS in hematopoiesis and immune functions in vertebrates, and provides the basis for the systematic genetic dissection of the PNS-hematopoietic axis in the future.