Recyclable and Robust Optical Nanoprobes with Engineered Enzymes for Sustainable Serodiagnostics
Recyclable and Robust Optical Nanoprobes with Engineered Enzymes for Sustainable Serodiagnostics
复制标题
DOI：
10.1002/adma.202306615
复制
发表时间：
2023-10-17
影响因子：
29.4
通讯作者：
Loeffler，Felix F.
中科院分区：
文献类型：
--
作者：
Liu，Yuxin;Wei，Zheng;Loeffler，Felix F.
可在室温下储存的可回收荧光测定法将极大地有利于生物医学诊断，因为它带来了可持续性和成本效益，特别是对发展中地区的护理点血清诊断而言。本文提出了一种总体策略，通过使用具有增强热/化学稳定性的工程酶来产生可回收的荧光探针，该探针在10次回收以及在高温（37°C保存10天）下保持出色的血清诊断性能（准确性>95%）。有了这三个突出的特性，可回收的荧光探针可以通过使用不同的酶来检测各种临床重要的生物标志物。
Recyclable fluorescence assays that can be stored at room temperature would greatly benefit biomedical diagnostics by bringing sustainability and cost‐efficiency, especially for point‐of‐care serodiagnostics in developing regions. Here, a general strategy is proposed to generate recyclable fluorescent probes by using engineered enzymes with enhanced thermo‐/chemo‐stability, which maintains an outstanding serodiagnostic performance (accuracy >95%) after 10 times of recycling as well as after storage at elevated temperatures (37 °C for 10 days). With these three outstanding properties, recyclable fluorescent probes can be designed to detect various biomarkers of clinical importance by using different enzymes.