Sensitive fluorescence detection of lysozyme using a tris(bipyridine)ruthenium(II) complex containing multiple cyclodextrins.
Sensitive fluorescence detection of lysozyme using a tris(bipyridine)ruthenium(II) complex containing multiple cyclodextrins.
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DOI：
10.1039/c5cc00428d
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发表时间：
2015-03
影响因子：
4.9
通讯作者：
Fan Zhang;Yingying Zhao;Hong Chen;Xiuhua Wang;Qiong Chen;P. He
中科院分区：
文献类型：
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作者：
Fan Zhang;Yingying Zhao;Hong Chen;Xiuhua Wang;Qiong Chen;P. He
一系列新的光活性金属环糊精与 ssDNA/适体结合后荧光强度增加，已被证明能够灵敏、选择性地检测溶菌酶。检测机制依赖于适体-溶菌酶复合物的形成，这会导致荧光强度降低。
A new series of photoactive metallocyclodextrins with increased fluorescence intensity upon binding with ssDNAs/aptamers has been demonstrated to sensitively and selectively detect lysozyme. The detection mechanism relies on the formation of an aptamer-lysozyme complex, which leads to reduction of fluorescence intensity.