Characterization of Epstein-Barr virus-related thymidine kinase induced in nonproducer cells by superinfection or chemical treatment.
Characterization of Epstein-Barr virus-related thymidine kinase induced in nonproducer cells by superinfection or chemical treatment.
复制标题
通过重复感染或化学处理在非生产细胞中诱导的 Epstein-Barr 病毒相关胸苷激酶的表征。
作者：
P. Mac Gabhann;K. Sugawara;Y. Ito
12- o - tetradecanoylphorbol13 -acetate和n-butyrate联合化学处理或P3HR-1病毒重复感染可有效诱导Raji和NC37 TK阴性突变细胞产生新型胸苷激酶（TK）。鼻咽癌患者血清中含有高滴度的eb病毒早期抗原抗体可使该诱导酶失活，而正常供体血清，即使含有高滴度的病毒衣壳抗原抗体，也对该酶无影响。此外，诱导酶对三磷酸胸腺嘧啶抑制相对不敏感。
Chemical treatment with a combination of 12-O-tetradecanoylphorbol-13-acetate and n-butyrate or superinfection with P3HR-1 virus effectively induced a novel thymidine kinase (TK) in Raji and NC37 TK-negative mutant cells. Sera from patients with nasopharyngeal carcinoma containing a high antibody titer against Epstein-Barr virus early antigen could inactivate this induced enzyme while sera from normal donors, even those containing a high titer of antibodies to virus capsid antigen, had no effect on the enzyme. Also, the induced enzyme was relatively insensitive to thymidine triphosphate inhibition.