Experimental herpes simplex keratitis. Corneal hydration, electrolyte content and structural changes.
Experimental herpes simplex keratitis. Corneal hydration, electrolyte content and structural changes.
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实验性单纯疱疹性角膜炎。
DOI：
10.1016/0002-9394(69)90714-4
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发表时间：
1969
影响因子：
4.2
通讯作者：
Diane L. Van Horn
中科院分区：
文献类型：
--
作者：
H. Edelhauser;Richard O. Schultz;Diane L. Van Horn
材料和方法所使用的接种物是来自组织培养物（Maben细胞，肺癌）中第六代的单纯疱疹病毒Walker株，以细胞悬浮液形式储存在-70°C。小鼠中的LD5 0为1.0 X 10" 516。将100 μl病毒悬浮液滴在36只体重在2.0至3.5 kg之间的白化兔子的每个角膜上。眼睛没有受到任何人为创伤，通过频繁的裂隙灯观察和角膜厚度的测量来跟踪疾病的过程。
MATERIALS AND METHODSThe inoculum used was the Walker strain of herpes simplex virus from the sixth passage in tissue culture (Maben cell, lung carcinoma) stored as a cell suspension at-70 C. The LD5 0 in mice was 1.0 X IO" 516. A 100 μΐ virus suspension was dropped on each of the corneas of 36 albino rabbits weighing between 2.0 and 3.5 kg. The cor neas were not subjected to any artificial trauma. The course of the disease was fol lowed with frequent slitlamp observations and measurements of corneal thickness. Each measurement of central corneal thick-