Blood and liver enzymes in rainbow trout (Salmo gairdneri Rich.) with emphasis on their diagnostic use: Study of CCl4 toxicity and a case of Aeromonas infection
Blood and liver enzymes in rainbow trout (Salmo gairdneri Rich.) with emphasis on their diagnostic use: Study of CCl4 toxicity and a case of Aeromonas infection
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虹鳟鱼 (Salmo gairdneri Rich.) 的血液和肝脏酶，重点是其诊断用途：CCl4 毒性研究和气单胞菌感染病例
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
C. Leray
中科院分区：
文献类型：
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作者：
J. Racicot;M. Gaudet;C. Leray
研究了每公斤鱼腹腔注射1.33 ml CCl 4的效果。进行了两个实验系列，并研究了10天（用稀释剂）和24小时（纯四氯化碳）的时间。测定了LDH、GOT、GPT、GR、GDH、CPK、G-6-氨基葡萄糖酸（G-6-glucose）和碱性磷酸酶（Alkaldehyde）。血液中所有酶的活性增加：LDH（4倍的控制），GOT（2倍），GPT（3倍），他们达到最大活性12小时后注射稀释的四氯化碳。还检查了肝脏中某些酶的水平。与纯四氯化碳，最大酶活性在血液中发生较早（6小时）。观察到GOT、GPT、LDH、GR和GDH增加6至10倍。组织学观察与这些酶的研究。
气单胞菌病的特点是破坏真皮，暴露的肌肉，并在肝脏中存在大量的瘀点，使我们能够检查自然诱导的组织损伤和血液中的酶水平之间的关系。测定了7种血液酶的水平，观察到LDH和CPK的最显著变化。使其浓度分别增加3至7倍。丙酮酸饱和试验表明，由于CCl 4中毒后观察到LDH，因此LDH可能来自肝脏。这种生物化学研究在鱼类研究中的贡献进行了评估。
The effect of one intraperitoneal injection of 1.33 ml of CCl4 per kg of fish was studied. Two experimental series were performed and studied for 10 days (with diluent) and 24 h (pure CCl4) periods. LDH, GOT, GPT, GR, GDH, CPK, G-6-Pase, and AlkPase were studied. The activity of all enzymes in blood increased: LDH (four times the control), GOT (two times), GPT (three times); they reached a maximal activity 12 h after injection of diluted CCl4. The levels of some enzymes were also examined in the liver. With pure CCl4, maximal enzyme activity in blood occurred earlier (6 h). A 6 to 10 times increase was observed for GOT, GPT, LDH, GR, and GDH. Histopathological observations were correlated with these enzymes studies.
An Aeromonas disease characterized by the destruction of the dermis, the exposure of the muscle, and by the presence of numerous petechiae in the liver enabled us to examine the relationships between naturally induced tissue damage and enzyme levels in blood. The levels of seven blood enzymes were determined and the most significant modifications were observed for LDH and CPK. which increased their concentration from 3 to 7 times respectively. A pyruvate saturation test demonstrated that LDH was probably from liver as it was observed after CCl4 poisoning. The contribution of such biochemical studies in fish research is evaluated.