Single Unlabeled Protein Detection on Individual Plasmonic Nanoparticles
Single Unlabeled Protein Detection on Individual Plasmonic Nanoparticles
复制标题
DOI：
10.1021/nl204496g
复制
发表时间：
2012-02-01
期刊：
影响因子：
10.8
通讯作者：
Soennichsen, Carsten
中科院分区：
文献类型：
--
作者：
Ament, Irene;Prasad, Janak;Soennichsen, Carsten
分析化学和生物学的最终检测极限是单分子。通常，采用荧光染料标记或酶扩增。这需要对分析物进行额外的标记，这会改变所调查的物种，从而影响生物过程。在这里，我们利用单个金纳米颗粒以极高的时间分辨率检测单个未标记的蛋白质。这允许在毫秒时间尺度上监测单个蛋白质结合事件的动态演变。这项技术甚至可以解决平衡覆盖波动问题，为未标记大分子的布朗动力学打开了一扇窗。因此，我们的方法可以在单分子水平上研究蛋白质折叠动力学、蛋白质吸附过程和动力学以及非平衡软物质动力学。
The ultimate detection limit in analytic chemistry and biology is the single molecule. Commonly, fluorescent dye labels or enzymatic amplification are employed. This requires additional labeling of the analyte, which modifies the species under investigation and therefore influences biological processes. Here, we utilize single gold nanoparticles to detect single unlabeled proteins with extremely high temporal resolution. This allows for monitoring the dynamic evolution of a single protein binding event on a millisecond time scale. The technique even resolves equilibrium coverage fluctuations, opening a window into Brownian dynamics of unlabeled macromolecules. Therefore, our method enables the study of protein folding dynamics, protein adsorption processes, and kinetics as well as nonequilibrium soft matter dynamics on the single molecule level.