Detection of Human Polyomavirus Proteins, T-Antigen and Agnoprotein, in Human Tumor Tissue Arrays
Detection of Human Polyomavirus Proteins, T-Antigen and Agnoprotein, in Human Tumor Tissue Arrays
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DOI：
10.1002/jmv.21514
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发表时间：
2010-05-01
影响因子：
12.7
通讯作者：
Khalili, Kamel
中科院分区：
文献类型：
--
作者：
Del Valle, Luis;Khalili, Kamel
人多瘤病毒JCV基因组在几种实验动物中的表达诱导多种神经源性肿瘤。病毒蛋白，T抗原和Agnoprotein，通过与几种肿瘤抑制蛋白和失调的信号传导通路相关，导致细胞增殖失控，从而促进JCV的肿瘤发生。此外，T抗原和Agnoprotein与DNA损伤和干扰DNA修复机制有关。在这项研究中，我们已经利用商业上可获得的各种来源的人类肿瘤的组织阵列，并证明了T-抗原和Agnoprotein在一些，但不是所有的神经和非神经来源的肿瘤中的表达。最值得注意的是，超过40%的人胶质母细胞瘤和超过30%的结肠腺癌表达病毒蛋白。在不存在病毒衣壳蛋白的情况下检测到病毒转化蛋白、T抗原和Agnoprotein，表明JCV在人类肿瘤的发生和/或进展中的作用。这些结果邀请进一步大规模调查的作用，多瘤病毒，特别是JCV在人类癌症的发病机制。医学病毒学杂志82：806- 811，2010。(C)2010 Wiley-Liss，Inc.
Expression of the human polyomavirus JCV genome in several experimental animals induces a variety of neural origin tumors. The viral proteins, T-antigen and Agnoprotein, contribute to the oncogenesis of JCV by associating with several tumor suppressor proteins and dys-regulating signaling pathways, which results in uncontrolled cell proliferation. In addition, T-antigen and Agnoprotein have been associated with DNA damage and interfering with DNA repair mechanisms. In this study, we have utilized commercially available tissue arrays of human tumors of various origins and demonstrated the expression of both T-antigen and Agnoprotein in some, but not all, tumors of neural and non-neural origin. Most notably, more than 40% of human glioblastomas and greater than 30% of colon adenocarcinomas express viral proteins. The detection of viral transforming proteins, T-antigen and Agnoprotein in the absence of viral capsid proteins suggests a role for JCV in the development and/or progression of human tumors. These results invite further large-scale investigation on the role of polyomaviruses, particularly JCV in the pathogenesis of human cancer. J. Med. Virol. 82:806-811,2010. (C) 2010 Wiley-Liss, Inc.