Functional prediction and characterization of Dip2 gene in mice
Functional prediction and characterization of Dip2 gene in mice
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小鼠 Dip2 基因的功能预测和表征
DOI：
10.1002/cbin.11106
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发表时间：
2019-04-01
影响因子：
3.9
通讯作者：
Zhu, Xiao-Juan
中科院分区：
文献类型：
--
作者：
Ma, Jun;Chen, Li;Zhu, Xiao-Juan
分离相互作用蛋白2（Disconnected interacting protein 2，DIP 2）是无脊椎动物和脊椎动物中高度保守的蛋白质家族，但其功能尚不清楚。本文总结了DIP 2家族成员基因序列和蛋白结构域的保守性，并预测它们可能在乙酰辅酶A（acetyl-CoA）合成中具有相似的功能作用。然后，我们使用最具特征的成员，断开相互作用蛋白2同源物A（DIP 2A），进行进一步的研究。DIP 2A是一种优先定位于线粒体的细胞质蛋白，其乙酰辅酶A合成酶活性已在体外得到证实。此外，过表达DIP 2A的HEK 293细胞中乙酰辅酶A的水平增加，这与其代谢相关功能一致。总之，这些数据丰富了dip 2基因的进化和功能表征，并为DIP 2的其他同源物的鉴定和应用提供了重要的见解。
Disconnected interacting protein 2 (DIP2) is a highly conserved protein family among invertebrates and vertebrates, but its function remains unclear. In this paper, we summarized the conservation of gene sequences and protein domains of DIP2 family members and predicted that they may have a similar functional role in acetyl‐coenzyme A (acetyl‐CoA) synthesis. We then used the most characterized member, disconnected interacting protein 2 homolog A (DIP2A), for further study. DIP2A is a cytoplasmic protein that is preferentially localized to mitochondria, and its acetyl‐CoA synthetase activity has been demonstrated in vitro. Furthermore, the level of acetyl‐CoA in HEK293 cells overexpressing DIP2A was increased, which is consistent with its metabolically related function. Together, these data enrich the evolutionary and functional characterization of dip2 genes and provide significant insights into the identification and application of other homologs of DIP2.