Hyaluronan-based scaffolds to tissue-engineer cartilage implants for laryngotracheal reconstruction.
Hyaluronan-based scaffolds to tissue-engineer cartilage implants for laryngotracheal reconstruction.
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基于透明质酸的支架用于组织工程软骨植入物以进行喉气管重建。
DOI：
10.1097/mlg.0b013e31811434ae
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
Dennis,JamesE
中科院分区：
文献类型：
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作者：
Weidenbecher,Mark;Henderson,JamesH;Tucker,HarveyM;Baskin,JonathanZ;Awadallah,Amad;Dennis,JamesE
目的：在喉气管重建（LTR）中获取软骨移植物时，供体部位的发病率（包括气胸）可能是一个相当大的问题。组织工程软骨可能为这个问题提供解决方案。研究设计：动物实验方法：18只新西兰白色兔接受LTR：12只接受自体组织工程软骨移植，6只作为阳性对照组，自体耳廓软骨。为了确定对植入部位的反应和对支架的任何潜在免疫反应的任何差异，将第二块工程化新软骨和非细胞负载的支架经喉插入兔的亚组中。分别于术后3、6、8、10、12周处死动物，检查喉功能。腔内可见光滑的非炎性瘢痕。在组织学上，自体耳廓软骨植入物表现出良好的整合，没有任何炎症或软骨降解的迹象。相反，所有的组织工程移植物和空支架显示出明显的非特异性异物反应迹象，导致新生软骨完全降解，无论是植入帕拉intralaryngeal.Conclusion：与Hyalograft C已成功应用于关节缺损修复相反，我们的研究结果表明，在兔子中，Hyalograft C如果植入内或喉内，会引发异物反应，导致软骨降解和可能的移植失败。这些发现表明，玻璃体移植物C的应用环境存在局限性。
Objectives:Donor site morbidity, including pneumothorax, can be a considerable problem when harvesting cartilage grafts for laryngotracheal reconstruction (LTR). Tissue‐engineered cartilage may offer a solution to this problem. This study investigated the feasibility of using Hyalograft C combined with autologous chondrocytes to tissue engineer cartilage grafts for LTR in rabbits.Study Design:Animal study.Methods:Eighteen New Zealand white rabbits underwent LTR: 12 rabbits received autologous tissue‐engineered cartilage grafts and 6 animals, serving as a positive control group, native auricular cartilage. To determine any differences in response to the site of implantation and any potential immune response to the scaffold, a second piece of engineered neocartilage and a non–cell‐loaded scaffold were inserted paralaryngeally into a subset of the rabbits. The rabbits were sacrificed 3, 6, 8, 10, and 12 weeks after the LTR and their larynx examined.Results:None of the 18 rabbits showed signs of respiratory distress. A smooth, noninflammatory scar was visible intraluminally. Histologically, the native auricular cartilage implants showed excellent integration without any signs of inflammation or cartilage degradation. In contrast, all tissue‐engineered grafts and empty scaffolds revealed marked signs of an unspecific foreign body reaction, leading to a complete degradation of the neocartilage, whether implanted para‐ or intralaryngeally.Conclusion:In contrast to the success with which Hyalograft C has been applied in articular defect repair, our results indicate that, in rabbits, Hyalograft C initiates a foreign body reaction if implanted intra‐ or paralaryngeally, leading to cartilage degradation and possible graft failure. These findings suggest limitations on the environment in which Hyalograft C can be applied.