Identification of representative genes of the central nervous system of the locust, Locusta migratoria manilensis by deep sequencing.
Identification of representative genes of the central nervous system of the locust, Locusta migratoria manilensis by deep sequencing.
复制标题
DOI：
10.1673/031.012.8601
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Zhang Z;Peng ZY;Yi K;Cheng Y;Xia Y
东亚飞蝗（Locusta migratoria manilensis（L.）（直翅目：蝗科）中枢神经系统（CNS）。在这项研究中，蝗虫CNS RNA的深度测序。共获得41,179个unigenes，平均长度为570 bp，其中5,519个unigenes的长度大于1,000 bp。与蝗虫Schistocerca gregaria Forsskåi EST数据库比较，发现9，069个unigenes保守，32，110个unigenes差异表达。共鉴定出15，895个unigenes，包括644个神经系统相关unigenes。在25，284个未知的unigenes中，通过过滤掉以前从蝗虫器官中获得的没有CNS的EST，发现9，482个对CNS具有特异性。蝗虫CNS与赤拟谷盗Tribolium castaneum（Herbst）（鞘翅目：拟步甲科）序列的匹配率最高（18%）。综合评估表明，在这项研究中产生的数据库是广泛的代表性的成年蝗虫中枢神经系统，提供全面的基因信息，在转录水平上，可以促进蝗虫中枢神经系统的研究，包括各种生理方面和农药靶标的发现。
The shortage of available genomic and transcriptomic data hampers the molecular study on the migratory locust, Locusta migratoria manilensis (L.) (Orthoptera: Acrididae) central nervous system (CNS). In this study, locust CNS RNA was sequenced by deep sequencing. 41,179 unigenes were obtained with an average length of 570 bp, and 5,519 unigenes were longer than 1,000 bp. Compared with an EST database of another locust species Schistocerca gregaria Forsskåi, 9,069 unigenes were found conserved, while 32,110 unigenes were differentially expressed. A total of 15,895 unigenes were identified, including 644 nervous system relevant unigenes. Among the 25,284 unknown unigenes, 9,482 were found to be specific to the CNS by filtering out the previous ESTs acquired from locust organs without CNS's. The locust CNS showed the most matches (18%) with Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) sequences. Comprehensive assessment reveals that the database generated in this study is broadly representative of the CNS of adult locust, providing comprehensive gene information at the transcriptional level that could facilitate research of the locust CNS, including various physiological aspects and pesticide target finding.