Eriocheir sinensis microRNA-7 targets crab Myd88 to enhance white spot syndrome virus replication
Eriocheir sinensis microRNA-7 targets crab Myd88 to enhance white spot syndrome virus replication
复制标题
中华绒螯蟹 microRNA-7 靶向螃蟹 Myd88 增强白斑综合症病毒复制
DOI：
10.1016/j.fsi.2018.05.028
复制
发表时间：
2018-08-01
影响因子：
4.7
通讯作者：
Ren, Qian
中科院分区：
文献类型：
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作者：
Huang, Ying;Wang, Wen;Ren, Qian
MicroRNAs （miRNAs）是在转录后水平调控基因表达的非编码rna。在这项研究中，研究了microRNA-7 （miR-7）在宿主-病毒相互作用中的功能。抗mirna寡核苷酸AMO-miR-7可通过过表达miR-7增强白斑综合征病毒（WSSV）的复制，通过下调miR-7抑制WSSV的复制。利用生物信息学方法预测miR-7的靶基因。结果显示，miR-7可靶向螃蟹髓样分化因子88 （Myd88）。当Myd88的表达被序列特异性siRNA敲除时，螃蟹的WSSV拷贝数显著增加。进一步的研究发现，Myd88、Tube或Pelle基因的敲低可抑制白细胞介素增强因子2同源物（ILF2）和白细胞介素16样基因（IL-16L）的表达。当ILF2被沉默时，IL-16L的表达被抑制。过表达miR-7抑制il - f2和IL-16L的表达。此外，当ILF2或IL-16L被沉默时，螃蟹的WSSV拷贝数增加。因此，在WSSV攻击过程中，miR-7的上调表达抑制了螃蟹宿主Myd88-ILF2-(IL-16L)信号通路，增强了WSSV的复制。我们的研究表明，WSSV在感染过程中利用螃蟹miR-7增强病毒复制。
MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression at the posttranscriptional level. In this study, the function of microRNA-7 (miR-7) in host-virus interaction was investigated. Replication of White spot syndrome virus (WSSV) was enhanced with the overexpression of miR-7 and inhibited with the downregulation of miR-7 by using anti-miRNA oligonucleotide AMO-miR-7. The target gene of miR-7 was predicted using bioinformatics methods. Results showed that crab myeloid differentiation factor 88 (Myd88) could be targeted by miR-7. When the expression of Myd88 was knocked down by sequence-specific siRNA, WSSV copies in crabs were significantly increased. Further findings revealed that knockdown of Myd88, Tube, or Pelle inhibited the expressions of interleukin enhancer-binding factor 2 homolog (ILF2) and interleukin-16-like gene (IL-16L). While ILF2 was silenced, IL-16L expression was inhibited. The overexpression of miR-7 inhibited the expressions of ILF2 and IL-16L. Moreover, when ILF2 or IL-16L was silenced, WSSV copies in crabs were increased. Thus, the up regulated expression of miR-7 during WSSV challenge suppressed the host Myd88-ILF2-(IL-16L) signaling pathway in crabs and enhanced WSSV replication. Our study indicated that WSSV utilized crab miR-7 to enhance virus replication during infection.