Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.)
Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.)
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DOI：
10.1007/s11240-009-9577-z
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发表时间：
2009-08
期刊：
影响因子：
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通讯作者：
P. Perera;V. Vidhanaarachchi;T. Gunathilake;D. Yakandawala;V. Hocher;J. Verdeil;L. Weerakoon
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文献类型：
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作者：
P. Perera;V. Vidhanaarachchi;T. Gunathilake;D. Yakandawala;V. Hocher;J. Verdeil;L. Weerakoon
椰子是一种异花授粉棕榈树，仅通过种子繁殖。组织培养是椰子唯一可用的无性繁殖方法。通过在含有 100 μM 2,4-二氯苯氧基乙酸 (2,4-D) 和 0.1% 活性炭的 CRI 72 培养基中培养 -4 阶段的未受精卵巢，获得一致的愈伤组织。使用 9 μM 噻苯隆 (TDZ) 可以改善愈伤组织。将胚发生愈伤组织传代培养到含有 66 μM 2,4-D 的体细胞胚胎发生诱导培养基上。在含有脱落酸 (ABA) 的 CRI 72 培养基上继代培养后，观察到体细胞胚胎生长发育不良。体细胞胚胎的成熟可以在没有生长调节剂的Y3培养基中实现。通过向含有 5 μM 6-苄基腺嘌呤 (BA) 的转化培养基中添加赤霉酸 (GA3) 来诱导体细胞胚胎的转化，而 2-异戊基腺嘌呤 (2iP) 则增加了植物再生的频率。 32 个培养子房总共产生 83 株幼苗。
Coconut is a cross pollinating palm, propagated only by seeds. Tissue culture is the only vegetative propagation method available for coconut. Consistent callogenesis was obtained by culturing unfertilised ovaries at -4 stage in CRI 72 medium containing 100 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1% activated charcoal. Callusing was improved by application of 9 μM thidiazuron (TDZ). Embryogenic calli were subcultured onto somatic embryogenesis induction medium containing 66 μM 2,4-D. Stunted growth was observed in the somatic embryos after subculture onto CRI 72 medium containing abscisic acid (ABA). Maturation of somatic embryos could be achieved in Y3medium without growth regulators. Conversion of somatic embryos was induced by adding gibberellic acid (GA3) to conversion medium containing 5 μM 6-benzyladenine (BA) while 2-isopentyl adenine (2iP) increased the frequency of plant regeneration. A total of 83 plantlets was produced from 32 cultured ovaries.