Distribution of lutein and zeaxanthin stereoisomers in the human retina
Distribution of lutein and zeaxanthin stereoisomers in the human retina
复制标题
DOI：
10.1006/exer.1996.0210
复制
发表时间：
1997-02-01
影响因子：
3.4
通讯作者：
Wang, WL
中科院分区：
文献类型：
--
作者：
Bone, RA;Landrum, JT;Wang, WL
黄斑色素立体异构体在人视网膜中的分布已经被绘制出来，并提出了一种解释非膳食类胡萝卜素，内消旋玉米黄质存在的途径。将成人神经视网膜切成以中央凹为中心的三个同心区域，并通过高效液相色谱法分析和纯化提取的类胡萝卜素。通过HPLC进一步分析每个组织样品的收集的玉米黄质级分的二氨基甲酸酯或二苯甲酸酯衍生物，以确定其立体异构体组成。对来自婴儿眼睛的整个视网膜进行了类似的分析。结果表明，相对于玉米黄质，叶黄素在成人神经视网膜中的浓度随着距中央凹的径向距离而增加，而中-玉米黄质的浓度降低。婴儿视网膜被发现有更多的叶黄素和更少的内消旋玉米黄质，相对于玉米黄质，比成人视网膜。在成人视网膜中也发现少量的（3S，3'S）-玉米黄质，特别是在黄斑中。有人提出，叶黄素和玉米黄质被运送到一个人的视网膜中，在他或她的血清中发现相同的比例。一些叶黄素然后转化为内消旋玉米黄质，主要是在黄斑中，通过一种在婴儿中比成人发育得更少的机制。(C)出版社：Academic Press Limited。
The distribution of macular pigment stereoisomers in the human retina has been mapped and a pathway to account for the presence of the non-dietary carotenoid, meso-zeaxanthin, is proposed. Adult neural retinas were cut into three concentric areas centered on the fovea, and the extracted carotenoids were analysed and purified by high-performance liquid chromatography. The dicarbamate or dibenzoate derivatives of the collected zeaxanthin fractions for each tissue sample were further analysed by HPLC to determine their stereoisomer composition. Whole retinas from infant eyes were similarly analysed. The results show that, relative to zeaxanthin, the concentration of lutein in the adult neural retina increases with radial distance from the fovea while that of meso-zeaxanthin decreases. Infant retinas were found to have more lutein and less meso-zeaxanthin, relative to zeaxanthin, than adult retinas. Small quantities of(3S, 3'S)-zeaxanthin were also found in the adult retina, particularly in the macula. It is proposed that lutein and zeaxanthin are transported into an individual's retina in the same proportions found in his or her blood serum. Some of the lutein is then converted into meso-zeaxanthin, primarily in the macula, by a mechanism which is less developed in infants than adults. (C) 1997 Academic Press Limited.