The effect of thermal treatment on the neuronal cell biocompatibility of SU-8
The effect of thermal treatment on the neuronal cell biocompatibility of SU-8
复制标题
DOI：
10.1016/j.mtcomm.2020.101073
复制
发表时间：
2020-09-01
影响因子：
3.8
通讯作者：
Halliez, Sophie
中科院分区：
文献类型：
--
作者：
Baetens, Tiffany;Begard, Severine;Halliez, Sophie
未来的体外生命科学研究，需要在原位功能超越普通的玻璃培养皿，可以通过使用微系统技术来制造生物微机电系统（BioMEMS）和芯片实验室（MEMS）。用于构造这些微系统的一种这样的材料是商业光致抗蚀剂SU-8。在这里，SU-8的神经元生物相容性检查作为其硬烘烤热处理的函数。SU-8表面是非图案化的大的均匀薄膜-这允许充分测试SU-8的生物相容性和细胞粘附。总共有75个SU-8样品使用旋涂和光刻技术制造的研究。我们观察到，180摄氏度的单一硬烘烤步骤2小时加上涂层（聚-D-赖氨酸+层粘连蛋白）足以解毒SU-8和促进原代皮层细胞粘附和存活长达28天的体外（DIV）。因此，这里描述的协议使SU-8表面与从原代神经细胞发育神经元网络兼容。
Future in vitro life science studies that require in situ functionalities-going beyond the common glass petri dish-can potentially be realized by using microsystems technologies to fabricate bio-microelectromechanical systems (BioMEMS) and laboratory-on-a-chip (LOC). One such material used to construct these microsystems is the commercial photoresist SU-8. Here, the neuronal biocompatibility of SU-8 is examined as a function of its hard-baking thermal treatment. The SU-8 surfaces were non-patterned large uniform thin films-this allowed the biocompatibility and cell adhesion of the SU-8 to be fully tested. In total, 75 SU-8 samples were fabricated for the study using spin-coating and lithography techniques. We observe that a single hard-baking step of 180 degrees C for 2 h coupled with a coating (poly-D-lysine + laminin) is enough to detoxify SU-8 and promote primary cortical cell adhesion and survival up to 28 days in vitro (DIV). Therefore, the protocol described here makes SU-8 surfaces compatible with the development of neuronal networks from primary neural cells.