Combined effects of diets with reduced calcium and phosphate and increased fluoride intake on vertebral bone strength and histology in rats.
Combined effects of diets with reduced calcium and phosphate and increased fluoride intake on vertebral bone strength and histology in rats.
复制标题
减少钙和磷酸盐饮食以及增加氟化物摄入量对大鼠椎骨强度和组织学的综合影响。
DOI：
10.1007/s002230010033
复制
发表时间：
2001
影响因子：
4.2
通讯作者：
Dunipace,AJ
中科院分区：
文献类型：
--
作者：
Turner,CH;Hinckley,WR;Wilson,ME;Zhang,W;Dunipace,AJ
摄入的氟化物会被合并到骨磷灰石中，并会影响骨骼的结构完整性。包括钙在内的其他离子的存在会影响肠道对氟化物的吸收，并影响氟化物进入骨骼。我们假设，低钙磷酸盐饮食和高氟摄入量将对椎骨强度产生独立的有害影响。我们测量了16周或48周给予不同氟化物和磷酸钙摄入量的对照饮食的大鼠的椎体生物力学特性和骨组织学。饮食设计是为了在保持钙磷比恒定的情况下产生轻微的钙缺乏。因此，钙缺乏伴随着成比例的磷酸盐缺乏。钙磷缺乏(正常的25%)仅在较年轻的大鼠中显著降低脊椎强度。随着大鼠年龄的增长，钙磷缺乏症的影响有所减轻。钙磷缺乏主要通过减少骨小梁体积来降低年轻大鼠的椎体强度。高氟摄入量(50 mg/L)显著降低椎体强度，但低氟摄入量对骨强度没有影响。与钙磷缺乏相反，高氟摄入对骨小梁体积没有影响，反而增加了未矿化类骨质的数量，尤其是在老年大鼠。氟化物对矿化的损害似乎是脊椎强度减弱的主要原因。氟化物对脊椎骨强度的不利影响不会因钙磷缺乏而放大。
Ingested fluoride is incorporated into bone apatite and can affect the structural integrity of bone. Fluoride absorption in the gut and incorporation into bone is affected by the presence of other ions, including calcium. We hypothesized that a low calcium phosphate diet combined with high fluoride intake would have independent detrimental effects on vertebral bone strength. We measured the vertebral biomechanical properties and bone histology for rats given controlled diets with varied fluoride and calcium phosphate intakes for either 16 or 48 weeks. Diets were designed to produce mild calcium deficiency while keeping Ca: P ratio constant. Hence calcium deficiency was accompanied by a proportional phosphate deficiency. Calcium phosphate deficiency (25% of normal) significantly diminished vertebral strength only in younger rats. As the rats grew older, the effects of calcium phosphate deficiency were mitigated. Calcium phosphate deficiency reduced vertebral strength in young rats primarily by reducing the trabecular bone volume. The highest fluoride intake (50 mg/L) significantly diminished vertebral strength, but there was no effect of lower fluoride intakes on bone strength. In contrast to calcium phosphate deficiency, high fluoride intake had no effect on trabecular bone volume but instead increased the amount of unmineralized osteoid, particularly in older rats. This impairment of mineralization by fluoride appeared to be the primary cause of the diminished vertebral strength. The detrimental effects of fluoride on vertebral bone strength were not amplified by calcium phosphate deficiency.